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MB ASCP Study Exam Questions 1 With Correct Answers 2024
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MB ASCP Study Exam Questions 1 With Correct Answers 2024
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MB ASCP Study Exam Questions 1 WithCorrect Answers 2024
Which vof vthe vfollowing vterms vrefers vto vthe vproportion vof vindividuals vaffected vby van
vautosomal vdominant vcondition vthat vdisplays vmanifestations?
A. vcodominance
B. vpenetrance
C. vexpressivity
D. vinfluence
E. vpassivity v- vcorrect vanswer.Answer vKey: vB vpenetrance
Feedback: vCertain vautosomal vdominant vconditions vare vvariably vexpressed vdue vto
vdifferences v in vpenetrance vand vexpressivity. vPenetrance vrefers vto vhow v people v with vthe
vgenetic vabnormality vmanifest vdisease, vand vexpressivity v refers vto vhow v severe vthose
vmanifestations vare.
Which vof vthe vfollowing vformulas vis vcorrect vfor vmelting vtemperature?
A. v4 v(A+T) v+ v2 v(G+C)
B. v4 v(G+C) v+ v2 v(A+T)
C. v4 v(G+A) v+ v2 v(T+C)
D. v4(G+T) v+ v2 v(A+C) v- vcorrect vanswer.B. v4 v(G+C) v+ v2 v(A+T)
All vof vthe vfollowing vmicroorganisms vare vtested vby vHybrid vCapture, vEXCEPT:
A. vCMV
B. vHPV
C. vC. vtrachomatis
D. vMRSA v- vcorrect vanswer.D. vMRSA v
Feedback: vMRSA vis vtested vby vReal-time vPCR
Mutations vin vwhich vof vthe vfollowing vgenes vresults vin vresistance vof vM. vtuberculosis vto
visoniazid vtreatment?
A. vpncA
B. vkatG
C. vRIF
D. vplcD
E. vvanA v- vcorrect vanswer.Answer vKey: vB vkatG
,Feedback: vMutations vin vthe vgene vkatG vcause visoniazid vresistance vin vM. vtuberculosis,
vmost vthese vmutations voccur vin vcodon v315. v
-RIF vgene vmutations vcause vrifampin vresistance,
-while vmutations vin vthe vpncA vgene vresult vin vpyrazinamide vresistance. v
-vanA vis va vgene vfound vin vS. vaureus vand vis vassociated vwith vresistance vto vvancomycin
RIF vgene vmutations vcause v- vcorrect vanswer.rifampin vresistance
mutations vin vthe vpncA vgene vresult vin v- vcorrect vanswer.pyrazinamide vresistance.
vanA vis va vgene vfound vin vS. vaureus vand vis vassociated vwith vresistance vto v- vcorrect
vanswer.vancomycin
Which vof vthe vfollowing vrepresents va vfull vturn vin vB-form vDNA?
A. v10 vbase vpairs
B. v3.4 vnm
C. v36 vdegrees
D. vA v& vB
E. vA, vB, vC v- vcorrect vanswer.Answer vKey: vD v10 vbp vand v3.4 vnm
Feedback: vB-form vDNA, vthe vonly vphysiologically vrelevant visoform, vhas va vperiodicity vof
v10 vbase vpairs vper vfull vturn. vThis vequates vto va v36 vdegree vrotation vfor veach vbase vpair.
vThe vlength vof v10 vconsecutive vbases vis v3.4 vnm.
What vis vthe vnumber vof vbase vpairs vthat veach vhistone vcore vcomplex vbinds?
A. v17
B. v54
C. v107
D. v146
E. v213 v- vcorrect vanswer.Answer vKey: vD v146
Feedback: vThe vcore vhistone vcomplex vis van voctamer vof v2 vmolecules veach vof vH2, vH3,
vH4, vand vH5. vThe vnet vpositive v charge vof vthe vprotein vcomplex vbinds vthe vnegatively
vcharged vDNA v with va vperiodicity v of v146 vbases. vThat vis, v146 vbase vpairs vof vDNA vcoil
varound vthe vhistone vcore v in va vsolenoid vconfiguration.
Preferred vmethod vfor velectroblotting vtransfer vis:
A. vAgarose vgel velectrophoresis
B. vCapillary velectrophoresis
C. vPAGE
D. vnone vof vthe vabove v- vcorrect vanswer.Answer vKey: vC vPAGE
polyacrylamide vgel velectrophoresis
Extracted vRNA vsamples vshould vbe vshipped
A. vfrozen, von vdry vice.
B. vat vambient vtemperature.
C. vin vEDTA vanticoagulant.
D. vspotted von vGuthrie vcards. v- vcorrect vanswer.Answer vKey: vA vfrozen, von vdry vice.
,This vtranslocation vis vassociated vwith vBurkitt's vlymphoma
A. vt(18;14)
B. vt(9;22)
C. vt(8;14)
D. vt(15;17) v- vcorrect vanswer.t(8;14)
Acute vpromyelocytic vLeukemia v(APL) vis vblamed vfor vthis vtranslocation:
A. vt(8;14)
B. vt(15;17)
C. vt(9;22)
D. vt(11;22) v- vcorrect vanswer.B. vt(15;17)
The vphysician vsuspects va vsingle-gene vdisorder vmay vbe vthe vcause vof vthose vclinical
vmanifestations. vA vblood vspecimen v was vthen vsent vto v your vclinical vlaboratory vfor
vmutation vscreening vin vthe v Huntington vgene. vWhich vof vthese vmethods v would vbest
vaccomplish vthis vtask.
A. vStandard vPCR
B. vPFGE
C. vRAPD-PCR
D. vMethylation-specific vPCR v- vcorrect vanswer.A. vStandard vPCR
What vtype vof vinheritance vpattern vis vcharacterized vby vaffecting vmales vin vevery vother
vgeneration?
A. vautosomal vdominant
B. vautosomal vrecessive
C. vmitochondrial
D. vX-linked vdominant
E. vX-linked vrecessive v- vcorrect vanswer.Answer vKey: vE vX-linked vrecessive
Feedback: vBecause vmales vare vhomozygous vfor vX, vthey vare vaffected vmore vfrequently
vthan vfemales, v who vare vmost voften vheterozygous vcarriers
Larger vfragments vof vDNA vare vbest vseparated von
A. v0.5% vagarose.
B. v5% vagarose.
C. v15% vpolyacrylamide.
D. v20% vpolyacrylamide. v- vcorrect vanswer.A. v0.5% vagarose.
What vis vthe vconcentration vin vpmol/ml, vof vhigh vmolecular vweight vDNA vwith va
vconcentration vof v412.5ug/ml, vgiven vthe vlength vof vthe vDNA vat v7250 vnt?
A. v17pmol/ul
B. v1.7pmol/ul
C. v0.017pmol/ml
D. v0.17pmol/ml v- vcorrect vanswer.Answer vKey: vD
Feedback: vpg v= v1x106 vug
7250nt/2 v= v3625bp
, 3625bp vx v660pg/pmol v= v2.4x106 vpg/pmol
412.5ug/mL vx v1pg/1x106 vug v= v.0004125pg/mL
.0004125pg/mL vx v1pmol/2.4x106pg v= v0.17pmol/mL
What vhappens vto vwhen vchromosomes vlose vtheir vtelomerase vactivity?
A. vit vwill vincrease vin vlength vafter vevery vcell vcycle
B. vit vwill vdecrease vin vlength vafter vevery vcell vcycle
C. vit vwill vremain vthe vsame vafter vevery vcell vcycle
D. vnone vof vthe vabove v- vcorrect vanswer.Answer vKey: vB vit vwill vdecrease vin vlength vafter
vevery v cell vcycle
Parentage vtesting vreports vmust vinclude vwhich vof vthe vfollowing vinformation?
A. vCorrelation vwith vmorphologic vfindings.
B. vIdentity vof vanalyst vcompleting vthe vtest.
C. vKilobase vsize vof valleles vfor vPCR.
D. vMean vmaternity vprobability vindex. v- vcorrect vanswer.Answer vKey: vB vIdentity vof vanalyst
vcompleting vthe vtest.
In van velectrophoresis vchamber, vthe vsample vloading vwells vare vpositioned vclosest vto vthe:
A. vcathode.
B. vvoltmeter.
C. velectrode vchamber.
D. vcasting vtray. v- vcorrect vanswer.Answer vKey: vA vcathode.
Which vof vthe vfollowing vtransitions vbest vdescribes vthe vcell's ventry vinto vthe vcell vcycle?
A. vS vto vM
B. vG2 vto vM
C. vG1 vto vS
D. vG1 vto vG2
E. vG1 vto vM v- vcorrect vanswer.Answer vKey: vC v- vG1 vto vS
Feedback: vThe vS vphase vmarks vthe vbeginning vof vthe vcell vcycle, vthough vas va vcycle vit vhas
vno vtrue vbeginning vor vend. v During vS vphase, vprotein vproduction vshifts vinto vhigh v gear vand
vmany vproteins vthat vare vrequired vfor vsubsequent vmitosis vare vsynthesized.
Star vactivity vrefers vto
A. vover-digestion.
B. vnonspecific vamplification.
C. vtemplate vdissociation.
D. vprehybridization vblocking. v- vcorrect vanswer.Answer vKey: vA vover-digestion.
Which vof vthe vfollowing vis vthe vbest vdefinition vof va vrestriction vfragment vlength
vpolymorphism v(RFLP)?
A. vdecrease vin vthe vnumber vof vpolymorphisms vdue vto vfragment vlength
B. vdecrease vin vfragment vlength vof vDNA vregardless vof vpolymorphism
C. vmultiple vpleomorphic vconfigurations vof vDNA
D. van valtered vlength vof vDNA vdue vto va vmutation vof va vrestriction vendonuclease vsequence
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