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MCB4304 Prep Exam 1 Questions & Answers 2024/2025

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MCB4304 Prep Exam 1 Questions & Answers 2024/2025 Operon - ANSWERSGroup of genes transcribed as a single polycistronic message. Controlled by a regulatory protein. Only in Prokaryotes. Regulon - ANSWERSGroup of genes transcribed as separate units. Controlled by a single regulatory protein. ...

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  • August 6, 2024
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MCB4304 Prep Exam 1 Questions &
Answers 2024/2025

Operon - ANSWERSGroup of genes transcribed as a single polycistronic message. Controlled by a
regulatory protein. Only in Prokaryotes.



Regulon - ANSWERSGroup of genes transcribed as separate units. Controlled by a single regulatory
protein.



lactose permease - ANSWERSlactose transporter. brings lactose into the cell



B-galactosidase - ANSWERScleaves glycosidic bond. Breaking Lactose into Glucose and Galactose



lacZ - ANSWERSbeta-galactosidase



lacY - ANSWERSlactose permease



Negative regulation of transcription (inducible) - ANSWERSRepressor binds to repressor binding site,
blocking transcription. Until an inducer binds repressor, together leave DNA, allowing transcription



Negative Regulation (repressible) - ANSWERSAporepressor must bind with co-factor in order to be able
to bind repressor binding site, then blocking transcription.



Positive Regulation - ANSWERSan activator protein binds activator binding site to recruit RNA Polymerase
(Transcriptase)



lacI - ANSWERSencodes the lac repressor



Complementation - ANSWERSGene added is the same as Mutation

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