Streamline Microbiology Lab Quiz 1--
BIO 250L Questions And Answers With
Verified Solutions (GRADED A+)
what is the objective of air exposure? - ANSWER✔✔ to demonstrate that
microorganism are around us
define the medium? - ANSWER✔✔ liquid or gel designed to expose bacteria
what are the two types if medium and what do they provide? - ANSWER✔✔ broth
and agar; they provide variable growth conditions in the form of growth and
stability
what three ways do you handle media? - ANSWER✔✔ make sure that the top is
on the bottom; plate is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
define agar - ANSWER✔✔ chemical derived from seaweed that solidifies into a
jelly-like substance and becomes a solid
what are two types of agar? - ANSWER✔✔ plates and slants
example of agar? - ANSWER✔✔ TSA--- trypticase soy agar
, what is the air exposure procedure? - ANSWER✔✔ open the plate to expose it to
the air for several minutes then seal with parafilm and place in an incubate at 22C
for 24 hours then 4C until the next week
what is the objective of a surface swab? - ANSWER✔✔ to demonstrate that their
are microorganism and can only kill some
what is sterilization and some examples? - ANSWER✔✔ when you kill all the
bacteria;
autoclave and radiation
what is autoclave? - ANSWER✔✔ example of sterilization when you use heat and
pressure
what is disinfection and an example? - ANSWER✔✔ only kills some or most of the
microorganisms; Lysol
when streaking plates, be sure to work close to the Bunsen burner why?? -
ANSWER✔✔ because hot air rises creating a convection current therefore all the
bacteria floats up causing your work no to be contaminated
cross hatch pattern is what and what is it used in? - ANSWER✔✔ surface swab
and zigzag long was and up and down
what is the objective of aseptic technique and transferring culture - ANSWER✔✔
to create a subculture of Staphylococcus Aureus
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