Biol 227 Lab Exam; Questions and Answers 100% Correct
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Course
Bio 227
Institution
Bio 227
Biol 227 Lab Exam; Questions and Answers
100% Correct
body of microscope Answer-arm and base
light source Answer-Projects light upwards through the diaphragm, the specimen, and
the lenses. Has on/off switch and intensity knob
eyepiece Answer-Contains the ocular lens
ocular lens Answer-magni...
Biol 227 Lab Exam; Questions and Answers
100% Correct
body of microscope Answer-arm and base
light source Answer-Projects light upwards through the diaphragm, the specimen, and
the lenses. Has on/off switch and intensity knob
eyepiece Answer-Contains the ocular lens
ocular lens Answer-magnifies the image 10x
total magnification Answer-objective lens x ocular lens
field of view Answer-The area visible through the microscope eyepiece
objective lens Answer-the part of a compound light microscope that is located directly
above the specimen and that magnifies the image of the specimen (4x, 10x, 40x, 100x)
working distance Answer-distance between objective lens and specimen
stage of microscope Answer-Holds the slide or object you are looking at. (move with x
and y knobs)
, Biol 227 Lab Exam; Questions and Answers
100% Correct
focus adjustment knobs Answer-course adjustment knob for larger focusing and fine
adjustment knob for precision focusing
condenser Answer-focuses light through the specimen
condenser focus knob Answer-raises and lowers the condenser relative to the stage
(vertical height adjustment)
diaphragm Answer-Regulates the amount of light on the specimen
aperture iris diaphragm lever (contrast lever) Answer-Adjust intensity of image in precise
increments; left increases intensity, right decreases intensity
field iris diaphragm Answer-controls the amount of light entering the condenser
interpupillary distance Answer-distance between eyepieces
diopter adjustment Answer-Useful as a means to change focus on one eyepiece so as to
correct for any difference in vision between your two eyes.
Using a microscope Answer-Clean lenses, eyepiece, and objectives with lense paper
, Biol 227 Lab Exam; Questions and Answers
100% Correct
turn on light to midlevel
Raise condenser to highest position
Place slide on stage and center
Using 100x objective, raise stage until slide almost touches
Find specimen with 4x
Rotate 10x and bring into focus with fine adjustment
Adjust interpupillary distant and diopter adjustments
Kohler illumination
Purpose of Kohler illumination Answer-Allows for high contrast and resolution with
reduced heat on the specimen
Kohler illumination Answer-Contrast level/aperture iris to middle
close field iris diaphragm, center the circle
lower condenser until polygon is clear
open field iris to edge of FOV
bright field Answer-dark specimen on bright background
dark field Answer-bright specimen on dark background (good for transparent specimen)
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