Microbiology Lab UTA - Final Exam; Questions and Answers % Correct
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Course
Micro UTA
Institution
Micro UTA
Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
How is Media made? Correct answer-5 g. T.S.A. and 125 mL Di water, cover flask with foil,
affix autoclave tape, autoclave, wait 10-20 min to cool, pour into petri-dish to thickness of lid. or
stop when covers bottom.
Agar Co...
Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
How is Media made? Correct answer-5 g. T.S.A. and 125 mL Di water, cover flask with foil,
affix autoclave tape, autoclave, wait 10-20 min to cool, pour into petri-dish to thickness of lid. or
stop when covers bottom.
Agar Correct answer-linear polysaccharide polymer derived from agarose in red algae.
What does agar do? Correct answer-increases solidity, exhibits hysteresis- gels and melts
at different temps. Melts above 85-90 and solidifies at 40-45.
What is liquid media? Correct answer-commonly called broths, doesn't contain agar, easy
to use, allows observation of growth patterns.
Advantages to using semi-solid media Correct answer-To test oxygen requirements, to
test motility.
Fastidious Correct answer-only grows in a fortified culture media under specific
conditions. The more _____________ the organism, the more specific ingredients a medium
must have. Uses defined media.
Why use solid media? Correct answer-The maintenance and growth of stock cultures, to
isolate single colonies, to ensure purity.
, Microbiology Lab UTA - Final Exam; Questions
and Answers % Correct
T.S.A. Plate Correct answer-Tryptic Soy Agar most common medium used in lab.
Undefined media that contains casein digest, soybean digest, NaCl, and agar.
What are the 4 biochemical families all living things are composed of? Correct answer-
Proteins, Carbohydrates, lipids, nucleic acids
How much water does a bacteria cell consist of? Correct answer-70%
Autoclave Correct answer-sterilizes with steam at 121* C, pressure at 15 PSI for at least
15 min. Tested weekly to daily.
Reservoir Correct answer-potential source of infection.
Normal commensal symbionts Correct answer-S. pneumoniae and s. aureus
Environmentally acquired microbes Correct answer-p. aeruginosa and b. cepacia
Human error in inoculation Correct answer-What is responsible for most irregularities in
agar slants?
Five I's Correct answer-inoculation, incubation, isolation, inspection, identification
Flocculence Correct answer-Clumpy colonial aggregates seen in broth media.
streptococcus
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