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Microbiology Question and answers correctly solved

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Microbiology Question and answers correctly solved Fungi Cells Eukaryotic internal organelles Cell walls = complex carbs / Chitin + Glucan + Mannose protein - Glucan is a Pharm target for Fungi - Also contain Ergosterol Yeast Single-celled fungi Replicate by budding Buds = Blastoconidi...

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microbiology question and answers correctly solved

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Microbiology
Fungi Cells
Eukaryotic internal organelles
Cell walls = complex carbs / Chitin + Glucan + Mannose protein
- Glucan is a Pharm target for Fungi
- Also contain Ergosterol

Yeast
Single-celled fungi
Replicate by budding
Buds = Blastoconidia
Tested for using: Morphology and Biochem
- API 20C AUX / DNA Techniques

Monomorphic Filamentous Fungi (Mold)
Fungi with Hyphae (filamentous cells)
- Cluster of Hyphae = Mycelium
- Septate Hyphae = have cross walls (septum)
- Nonseptate Hyphae = Rapid Irregular Growth / Can be fatal
Produce airborne spores (Conidia)
Mushrooms are the fruiting bodies of filamentous fungi
Tested for using: Antigens / DNA probes

,Dimorphic fungal pathogens
Convert from Filamentous -> Yeast form when they enter the body
- In the cold, it's mold (at 37º becomes yeast)
Major Respiratory pathogenic fungi in US
- Histoplasma (Tested for with blood smear)
- Blastomyces
- Coccidiodes
- Sporothrix

Histoplasma capsulatum
Dimorphic pathogen
Inhaled from environment -> Lung macrophages
Intracellular growth / Systemic Circulation
Resistant to lysosomes

Cell Types of Pathogens
Bacteria = prokaryotic
Viruses = non-cellular
Fungi = eukaryotic
Protozoa / Helminths = Parasites (Eukaryotic)
Prions = non-cellular

,Bacteria
Cell wall = Peptidoglycan
Plasma Membrane without sterol
Contain Appendages
Cytosol = no membrane bound organelles
dsDNA / Circular Chromosome (Nucleoid)
70S Ribosomes (30S + 50S)

Parasites
Ectoparasites = live on skin / hair
Endoparasites = live in the host
Protozoans = Protista
- Unicellular (Amoeba / Flagellate / Ciliates / Sporozoa)
Metazoans = Animals (Insects / Worms)

Prions
Non-cellular / infectious proteins
Normal protein = PrPc
PrPsc = prion
- converts PrPc -> PrPsc
- results in Neurologic Disease

Strict Pathogens

, Microbes that will always cause a disease (even if healthy)
Includes:
- Tuberculosis
- Gonorrhea
- Tularemia
- Malaria
- Rabies

Sterile Sites in Body
Brain / CNS
Inner / Middle Ear
Lower Resp Tract
Kidneys / Ureters / Bladder
Uterus / Endometrium

Naked Virus
Protein Capsid + Nucleic acid genome (can be RNA or DNA)

AD
Enveloped Virus
Envelope + Matrix + Envelope Proteins (Spikes) + DNA/RNA

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