MICROBIOLOGY LABORATORY MANUAL TEST 3 QUIZ WITH VERIFIED Q&A 2024/2025
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Course
MICROBIOLOGY LABORATORY
Institution
MICROBIOLOGY LABORATORY
MICROBIOLOGY LABORATORY
MANUAL TEST 3 QUIZ WITH
VERIFIED Q&A 2024/2025MICROBIOLOGY LABORATORY
MANUAL TEST 3 QUIZ WITH
VERIFIED Q&A 2024/2025MICROBIOLOGY LABORATORY
MANUAL TEST 3 QUIZ WITH
VERIFIED Q&A 2024/2025MICROBIOLOGY LABORATORY
MANUAL TEST 3 QUIZ WITH
VERIFIED Q&A 2024/2025MICROBIOLOG...
MICROBIOLOGY LABORATORY
MANUAL TEST 3 QUIZ WITH
VERIFIED Q&A 2024/2025
discarding materials - ANSWER>>>autoclave before disposal:
placed in the discard pile
broken glassware - ANSWER>>>contaminated glass is placed in the large metal can on the discard table
non-contaminated glass is placed in the large cardboard "broken glass" box
spills - ANSWER>>>if you spill anything in the lab, inform the instructor or TA so that they can assist you
in proper clean-up. If culture is spilled on your clothing or belongings, they may require decon to assure
your safety.
parts of a microscope - ANSWER>>>eyepiece, objective lens, fine adjustment know, course adjustment
knob, stage, stage clips, diaphragm
ocular lenses (eye pieces) - ANSWER>>>lenses closest to the eye, typically magnify 10X
ocular width adjustment - ANSWER>>>adjust for your eye span
revolving nosepiece - ANSWER>>>can be rotated to change from one objective to another
stage adjustment - ANSWER>>>moves the slide on two horizontal planes: back and forth and side to side
condenser lens - ANSWER>>>condenses light waves into a pencil-shaped cone, thereby preventing the
escape of light waves. It's ideal position is just beneath the stage.
condenser knob - ANSWER>>>Raises and lowers the condenser. For this course the condenser should
normally be all the way up.
light control - ANSWER>>>turns the light source on and off. The degree it is turned on determines the
intensity of light.
, light source - ANSWER>>>Projects light upwards through the diaphragm, the specimen, and the lenses
base - ANSWER>>>supports the microscope
coarse adjustment knob - ANSWER>>>moves the stage up and down rapidly for purposes of approximate
focusing. Should only be used when focusing using low power objectives (4X & 10X)
fine adjustment knob - ANSWER>>>moves the stage up and down very minutely for purposes of
definitive focusing. When viewing with 40X or 100X objectives, use only the fine, not the coarse
adjustment knob
stage - ANSWER>>>Supports the slide being viewed
compound microscope - ANSWER>>>a light microscope that uses more than one lens to magnify an
object
magnification - ANSWER>>>An increase in the apparent size of an object
microscope resolution - ANSWER>>>ability of a lens to separate or distinguish small objects that are
close together
parfocal - ANSWER>>>Property of microscope which allows objectives to be changed without having to
refocus
working distance - ANSWER>>>the distance between the specimen and the objective lens
refractive index - ANSWER>>>a measure of the light-bending ability of a medium
oil immersion - ANSWER>>>This objective of the microscope is used for observing bacteria,WBC
differential count and RBC morphology
Determine total magnification - ANSWER>>>ocular lens x objective lens
wet mount - ANSWER>>>a glass slide holding a specimen suspended in a drop of liquid (as water) for
microscopic examination
fixed smear - ANSWER>>>helps the cells adhere to the slide so that they can be stained.
Purpose of heat fixing is to kill the organisms without serious distortion. They adhere better to the slide
and also take up dye more easily.
phase contrast microscope - ANSWER>>>light microscope that enhances contrast; useful in examining
living, unstained cells
brightfield microscope - ANSWER>>>a microscope that allows light rays to pass directly to the eye
without being deflected by an intervening opaque plate in the condenser
simple stain - ANSWER>>>adds dark color specifically to cells, but not to the external medium or
surrounding tissue
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