Exam (elaborations)
DAT LAB STUDY QUESTIONS AND ANSWERS WITH SOLUTIONS 2024
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DAT LAB STUDY QUESTIONS AND ANSWERS WITH SOLUTIONS 2024
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DAT LAB STUDY QUESTIONS ANDANSWERS WITH SOLUTIONS 2024
what is the purpose of performing the DAT procedure? - ANSWER used to determine if red cells have
been coated in vivo with immunoglobulin, complement or both.
what does a positive DATE indicate? - ANSWER indicates that red cells are coated with protein in-vivo
what 3 reagents may be used in the DAT procedure? - ANSWER polyspecific IgG, C3d
monospecific IgG or C3d
what is the sample of choice for the DAT? Why? - ANSWER whole blood collected in an EDTA tube
because it prevents in-vitro fixation of complement.
the DAT is used to assist in hemolysis associated with what 4 clinical conditions: - ANSWER HDFN
autoimmune hemolytic anemia
red cell sensitization
transfusion rxns
what 3 items in the pt history are crucial in determining the clinical significance of a positive DAT? -
ANSWER pts diagnosis
transfusion and pregnancy hx
recent/current medications
state the principle of the DAT procedure - ANSWER the detection of abs that attached to red cells in-vivo
what ab class is involved in HDFN? - ANSWER IgG
what is the source of the specimen used for the DAT on newborns? - ANSWER cord blood sample
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