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Test bank For Clinical Chemistry Review 9th Edition by Bishop Fody Questions & Answers with rationales
Test bank For Clinical Chemistry Review 9th Edition by Bishop Fody Questions & Answers with rationales
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Test bank For Clinical Chemistry Review9th Edition by Bishop Fody Questions &
Answers with rationales
Spectrophotometry principle
chemical reaction produces colored substance that absorbs light of a specific wavelength.
Amount of light absorbed is directly proportional to concentration of analyte.
Spectrophotometry components
light source, monochromator, cuvette, photodetector, readout device.
AA spectrophotometry principle
measures light absorbed by ground-state atoms
AA spectrophotometry component parts
hollow cathode lamp, atomizer flame, mixing chamber, chopper, monochromator, detector,
readout device
Fluorometry principle
atoms absorb light of specific wavelength and emit light of longer wavelength (lower energy)
fluorometry components
light source (mercury or xenon), primary monochromator, sample holder, secondary
monochromator, detector, readout device.
chemiluminescence principle
chemical rxn that produces light. usually involves oxidation of luminol, acridinium esters, or
dioxetanes
doesn't require excitation radiation or monochromators like fluorometry. Very sensitive. Used for
immunoassays
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