GESLAAGD IN EERSTE ZIT!!! Biochemie I wordt gegeven in 2e bachelor Diergeneeskunde, UGent door prof. Meyer.
Deze samenvatting bevat zowel de voorbereiding, het mechanisme (deel 1 en 2) als de controle en regeling van de krebs.
Bij de voorbereiding moet er niets zelf aangevuld worden. Bij het mechan...
• = pyruvaat => acetyl-CoA + CO2 via oxidatieve decarboxylatie
• Via 5 coënzymen: TPP → lipoamide → HS-CoA → FAD → NAD+
1. OXIDATIEVE DECARBOXYLATIE VIA E1
• Coënzyme TPP
• acetyl + 2 e•-transfer via OH*-ethyl-TPP,
afsplitsingCO2
• H+ tijdelijk gebonden
2. REDOX REACTIE VIA E1
• doel= recyclage TPP van E1 door oxidatie
• reductie via H- van coënzyme lipoamide van E2
• acetyl-transfer via acetyl-dihydrolipoamide
3. TRANSFER REACTIE VIA E2
• acetyl-groep van dihydrolipoamide naar HS-CoA
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