(MB) ASCP Study Exam Questions with 100% correct answers 2024
7 views 0 purchase
Course
ASCP S
Institution
ASCP S
What is metagenome? correct answersSampling the genome sequence of a community of organisms.
Polymorphism involved for Irinotecan? correct answersUGT1A1
NGS related to deletions and insertions, coverage, depth? correct answers-Paired-end reads for detection of indels
-Coverage is how much of...
What is metagenome? correct answersSampling the genome sequence of a community of organisms.
Polymorphism involved for Irinotecan? correct answersUGT1A1
NGS related to deletions and insertions, coverage, depth? correct answers-Paired-end reads for
detection of indels
-Coverage is how much of your target has been read
-Depth is how many times a unique position has been covered
Polymerase for lagging strand? correct answersEuk - DNA Pol Alpha
Pro - DNA Pol I
Polymerase for leading strand? correct answersEuk - DNA Pol Delta and DNA Pol Epsilon
Pro - DNA Pol III
Polymerase used in LAMP? correct answers-Loop-mediated Isothermal Amplification utilizes Bst
Polymerase
-Has helicase activity and activity at annealing/elongating temps
Highest resistance gene in enterococcus? correct answersvanA has the highest resistance in
enterococcus
Resistance in mecA gene in MRSA codes for? correct answersmecA responsible for antibiotic resistance
in S. aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam antibiotics like
penicillin.
Important in gene expression, defense in eukaryotes? correct answersPerson guessed mRNA - gene-
expression and codes for proteins that bind to DNA to promote/inhibit expression.
,Donor/Recipient - what's informative loci? correct answersNo matching alleles is informative, you can
tell D from R; non-informative would be both D&R matching.
Bond between DNA and histones? correct answersIonic - DNA is negative and histones are positive
Amino Acids that make up histones? correct answersArginine and Lysine - positively charged amino acids
Multiplex modifications for low yield? correct answersPerson guessed KCl.
Increasing Mg2+ could increase yield.
Decreasing stringency increases yield, but increases chances of mispriming
How does an amplification control help? correct answersDifferentiates a true negative from a false
negative
Amplification controls have a target that should always amplify, even if your sample does not amplify
Best molecular assay for Cystic Fibrosis? correct answersCF is caused by a 3b deletion: delF508
RFLP, PCR and Southern Blot are used.
, What to use FISH for: correct answersPWS/AS can be detected with FISH
Gaucher - Sequencing or enzyme activity screening
FXS - RFLP, PCR, SSCP
TS - Sequencing or enzyme activity screening
How often should a competency be given/done for someone in a complex lab? correct answersAnnually
Important in Final Lab Report in CLIA? correct answersLab director signature?
Neurotoxin in gel components? correct answersEthidium Bromide
What phenomenon is observed in EtBr in gel electrophoresis? correct answersProducts slowed because
EtBr alters DNA shape, charge, flexibility
Heteroduplex correct answersdsDNA where the complement strands are not completely complementary
MALDI question something about how it works? correct answersMatrix-assisted laser
desorption/ionization, blasts a surface with sample to produce positive ions that can be detected.
MALDI-Tof question? correct answersSame as MALDI, but used time of flight (TOF) to differentiate
between ions. Lighter ions travel faster.
NGS reaction order? correct answersFragmentation,
End Repair,
Adapter Ligation
Amplification
Sequencing
HIV processing order? correct answersPlasma separation, Extraction, RNA Modification, Amplification
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller QUILLSKY. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $16.49. You're not tied to anything after your purchase.
