FRET correct answers(Fluorescent Resonance Energy Transfer)
Uses TWO distinct probes to generate a signal.
probe 1: DONOR 5'
probe 2: REPORTER 3'
both anneal onto one strand of amplicon: SIGNAL.
When does a SYBR green probe generate a signal during PCR? correct answersExtension
When does ...
FRET correct answers(Fluorescent Resonance Energy Transfer)
Uses TWO distinct probes to generate a signal.
probe 1: DONOR 5'
probe 2: REPORTER 3'
both anneal onto one strand of amplicon: SIGNAL.
When does a SYBR green probe generate a signal during PCR? correct answersExtension
When does a MBG probe generate a signal during PCR? correct answers(minor groove binder) Annealing
When does a molecular beacon probe generate a signal during PCR? correct answersAnnealing
When does a TaqMan probe generate a signal during PCR? correct answersExtension (chomp chomp)
When does a Scorpion probe generate a signal during PCR? correct answersExtension
Which technique employs the amplification of the signal resulting from probe:target hybridization rather
than by amplifying the target or the probe? correct answersbranched DNA analysis (BDA)
It's performed by capturing a target nucleic acid onto a microtiter plate and then adding an amplifier
probe to greatly increase the signal resulting from the captured nucleic acid.
SIGNAL AMPLIFICATION TECHNIQUES correct answersBranched DNA analysis
Hybrid Capture Assays
Cleavage-Based Amplification
Cycling Probes
, Branched DNA analysis correct answersSIGNAL AMPLIFICATION
Capture probes immobilize target, extender probes bind to target, amplifiers give off loads of signal.
DNA:RNA:Ab:WALL. Secondary Abs bind to alkaline phosphatase generates signal in the presence of
chemiluminescent substrate.
Cleavage Based Amplification correct answersSIGNAL AMPLIFICATION
kind of target amplification..? amount of signal directly related to the amount of target molecules.
Cycling Probe correct answersSIGNAL AMPLIFICATION
produces fluorescence only when the RNA probe binds to the DNA template. RNA/DNA hybrid formed is
a substrate for RNAse H, which digests the RNA and releases the reporter dye from the quencher.
PROBE AMPLIFICATION TECHNIQUES correct answersLCR (Ligase chain reaction) - generates a signal by
repeated ligation of probes complementary to specific sequences in the template.
SDA (strand displacement amplification) - isothermal amplification; amplifies probe product without
restriction site due to incorporated modified nucleotides).
QB replicase (QB) - probes bound to template are amplified by QB replicase.
Bisulfite Sequencing correct answersModified chain terminator method specifically designed to detect
methylated nucleotides.
SSCP correct answersSingle strand conformational polymorphism - it's a screening test in which the exact
nature of a mutation does not need to be known in order to detect the presence of the mutation.
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