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Gel Electrophoresis Questions & Verified Answers
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Gel Electrophoresis Questions & Verified Answers
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Gel Electrophoresis Questions & Verified AnswersWhat is the purpose of gel electrophoresis? Right Ans - To separate DNA
fragments based on their size using electricity
What does "phoresis" mean? Right Ans - To transmit (move location,
migrate)
What three gel ingredients are required for gel electrophoresis? Right Ans -
Agarose, buffer, and distilled H2O
What is agarose? Right Ans - A sugar found in seaweed
What is a characteristic of agarose? Right Ans - It is gelatin-like
What purpose does agarose serve in gel electrophoresis? Right Ans - It is
the matrix (obstacle course) for DNA fragments (it separates them by size)
What is the buffer? Right Ans - A salt solution
What purpose does the buffer serve in gel electrophoresis? Right Ans - It
maintains pH (at 7 which is neutral) and conducts electricity which allows the
DNA fragments to migrate through the agarose gel
What purpose does the distilled H2O serve in gel electrophoresis? Right
Ans - It is the solvent
Why does the H2O need to be distilled? Right Ans - It contains no chlorine
or other contaminants that could damage DNA
How is the agarose power measured? Right Ans - On an electronic balance,
after zeroing out the weighing tray
How is the distilled H2O measured? Right Ans - In a graduated cylinder
(measured to the meniscus)
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