PTC Lab Comprehensive Exam Questions And Complete Detailed Answers.
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Course
PTC.
Institution
PTC.
Purpose of this lab - correct answer Analyze our own DNA to see what form of TAS2R38 (taster gene): heterozygous, homozygous (dominant or recessive)
What did we use to extract DNA from us? - correct answer ...
Purpose of this lab - correct answer Analyze our own DNA to see what form of
TAS2R38 (taster gene): heterozygous, homozygous (dominant or recessive)
What did we use to extract DNA from us? - correct answer Saline solution
Importance of saline solution - correct answer Salt neutralizes the negative
charge of the sugar phosphate backbone of DNA --> causes DNA to become less hydrophilic
Scraped away cells from mouth
Centrifuge - correct answer device to separate substances by spinning them at
high speed
What happens when we spun the centrifuge after using the saline solution? - correct answer
makes the heavier molecules move to the bottom of the test tube; pushed cells to the bottom which
formed a pellet (will be used for DNA extraction)
After centrifuging the 1st time, what did we do - correct answer added lysis
buffer (red) to resuspend the cell
lysis buffer - correct answer solution (which had proteinase K) used to break
the cell membrane and release cell contents (DNA)
why did we do a water bath for 55°C? - correct answer incubate the cell
solution during cell lysis;
- Optimal temperature for the enzyme Proteinase K to actively destroy cellular machinery.
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