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Pipetting and Gel Electrophoresis Lab 1 with results 2024
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Pipetting and Gel Electrophoresis Lab 1 with results 2024
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Pipetting and Gel Electrophoresis Lab 1 with results 2024Dispense RD onto the laminated sheet by doing the following: - answer > a. Place
the pipette tip over the 20.0 μL circle.
b. Use your thumb to press the plunger to the first stop position and then
press down to the second stop.
LAB TECHNIQUE: When dispensing liquid from the micropipette, press the
plunger to the first stop to dispense most of the liquid and then press the
plunger to the second stop in order to dispense the last little bit.
c. With the plunger still depressed, pull the pipette out of the tube—this
prevents you from accidentally pulling the liquid back into the tip.
What is the importance of micropipettes and gel electrophoresis in the genetic
engineering process? - answer > Micropipettes are used to transfer very small and
exact amounts of liquid-gel electrophoresis is used to separate and identify
plasmids and short linear pieces of DNA
what year marked a major breakthrough in medicine where scientists were able to
induce bacterial cells to make human insulin by inserting human DNA into the
cells. - answer > 1978
MATERIALS INCLUDE: - answer > -A plastic microfuge tube rack with a
microfuge tube of red dye solution (
RD)
-P-20 micropipette (measures 2.0-20.0 μL)
-Tip box of disposable pipette tips
-Laminated micropipette practice sheet
-Waste container for used tips and microfuge tubes (will be shared among groups)
, SAFETY EQUIPTMENT: - answer > -All appropriate safety precautions and attire
required for a science
laboratory should be used, including safety goggles. Please refer to your
teacher's instructions.
-Wash your hands well with soap after completing the lab.
how to increase and decrease volume - answer > Turn the plunger button on the
top of the micropipette clockwise—to the
right—to decrease the volume, or counterclockwise—to the left—to increase
the volume.
The display window of a micropipette shows______ - answer > how much fluid it
will load and dispense
Each group member will pipette _____drops of different volumes onto the sheet. -
answer > five
Pipetting consists of two parts: - answer > loading the liquid into the micropipette,
and dispensing the liquid from the micropipette.
Load the micropipette with 20.0 μL of RD by doing the following: - answer > a.
Set the micropipette to 20.0 μL.
b. Open the tip box. Lower the micropipette onto a tip and press down
firmly (do not touch the tip with your fingers). Close the box when done.
c. Bring the micropipette and the RD tube to eye level.
d. Use your thumb to press the plunger to the first stop position, which is
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