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Bio 181 - Prokaryotic Gene Regulation Homework

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  • August 17, 2024
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Chapter 18



BIO 181: Chapter 18 In class Activity Prokaryotic Gene Regulation

1. Match the following vocabulary terms to their correct definition.


__C___Feedback inhibition B. Process by which DNA is converted into RNA
C. The product of a metabolic pathway inhibits its
__L___Promoter
own production, when the product is in high
___B__Transcription concentration.
D. In a lac operon, a protein that binds to DNA and
__E___Operator
stimulates transcription.
__F___Operon E. Often found in the promoter, the switch that
___A__Repressor controls RNA polymerase access to the DNA.
F. Stretch of DNA that consists of the promoter
__G__Corepressor and the genes required for protein production.
__H___Inducer G. A small molecule that cooperates with the
repressor protein to switch the operon off.
__K___Regulatory Gene H. In the lac operon this molecule inactivates the
__I___Repressible operon repressor.
I. The type of operon that is “always” on but can
__J___Inducible operon be inhibited under certain conditions.
__D___CAP J. The type of operon that is “always” off but can
be stimulated under certain conditions.
K. This gene produces the repressor.
L. Region on the DNA that the RNA polymerase
binds to.

A. Binds to the operator to prevent transcription.


2. Label the following regions of the operon shown. Promoter, RNA polymerase, operator, genes, mRNA,
polypeptides, regulatory gene, and repressor.




Op mRNA
Repressor Polypeptides


Genes

, 3. Determine if the 2 images shown are of a repressible operon or an inducible operon.
Determine which image is an example of gene expression and which is of gene inactivation.

Picture 1 is an inducible operon while the picture 2 is a repressible operon.

4. Determine if the 2 images
shown are of a repressible
operon or an inducible operon.

Determine which image is an
example of gene expression and
which is of gene inactivation.

Picture 2 is an inducible operon while picture 1 is a repressible operon.



5. If a particular operon encodes enzymes for making an essential amino acid and is regulated like the trp
operon, then
A. the amino acid inactivates the repressor.
B. the repressor is active in the absence of the amino acid.
C. the amino acid acts as a corepressor.
D. the amino acid turns on transcription of the operon.

6. What would occur if the repressor of an inducible operon were mutated so it could not bind the operator?
A. irreversible binding of the repressor to the promoter
B. reduced transcription of the operon’s genes
C. buildup of a substrate for the pathway controlled by the operon
D. continuous transcription of the operon’s genes

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