Urinalysis and Body Fluids Exam With Verified Questions and Answers
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Course
Urinalysis and Body Fluids
Institution
Urinalysis And Body Fluids
Urinalysis and Body Fluids Exam With
Verified Questions and Answers
1. After receiving a 24-hour urine for quantitative total protein analysis, the technician must first:
a: subculture the urine for bacteria
b: add the appropriate preservative
c: screen for albumin with a dipstick
d: measure ...
Urinalysis and Body Fluids Exam With
Verified Questions and Answers
1. After receiving a 24-hour urine for quantitative total protein analysis, the technician must first:
a: subculture the urine for bacteria
b: add the appropriate preservative
c: screen for albumin with a dipstick
d: measure the total volume - answer✔✔d: measure the total volume; Measure the total volume
of the sample before removing an aliquot. To calculate the total protein, measure the protein of
an aliquot to learn the mg/dL. Then multiply that answer by the number of dL in the 24-hour
collection.
False results in urobilinogen testing may occur if the urine specimen is:
a: exposed to light
b: adjusted to a neutral pH
c: cooled to room temperature
d: collected in a nonsterile container - answer✔✔a: exposed to light; Urobilinogen is degraded
by light.
A clean-catch urine is submitted to the laboratory for routine urinalysis and culture. The routine
analysis is done first, and 3 hours later, the specimen is sent to the microbiology department for
culture. The specimen should:
a: be centrifuged and the supernantant cultured
b: be rejected due to the time delay
c: not be cultured if no bacteria are seen
d: be processed for culture only if nitrite is positive - answer✔✔b: rejected due to the time delay;
It is common practice to share samples between the microbiology department and urinalysis.
Ideally, the culture is set up first to prevent contamination. If that is not feasible timewise, the
sample should be aliquotted using aseptic technique, and refrigerated until it can be cultured.
which of the following urine results is most apt to be changed by prolonged exposure to light?
a: pH
b: protein
c: ketones
d: bilirubin - answer✔✔d: bilirubin; Bilirubin and urobilinogen are degraded by light, the other
analytes will not be affected.
Urine samples should be examined within 1 hour of voiding because:
a: RBCs, leukocytes and casts agglutinate on standing for several hours at room temperature
b: urobilinogen increases and bilirubin decreases after prolonged exposure to light
c: bacterial contamination will cause alkalinization of the urine
d: ketones will increase due to bacterial and cellular metabolism - answer✔✔c: bacterial
contamination will cause alkalinization of the urine; Evaluate each statement. A) is incorrect
because these cells don't agglutinate, b) is partially correct, but urobilinogen decreases in light.
C) is true because bacterial overgrowth does lead to an alkaline urine. D) is false because ketones
are produced by fat metabolism in the patient
MLS 6. The following results were obtained on a urine specimen at 8:00 AM: pH=5.5,
protein=2+, glucose =3+, ketones= 3+, blood= negative, bilirubin=positive, nitrite=positive
If this urine was stored uncapped at 5 degrees C without preservation and retested at 2 PM,
which of the following test results would be changed due to these storage conditions?
a: glucose
b: ketones
c: protein
d: nitrite - answer✔✔b: ketones; The sample is mistreated by being uncapped. It is refrigerated,
which will prevent bacteria from reproducing(so D is incorrect), and from metabolizing
glucose(so A is incorrect). Ketones can evaporate, but protein will not.
7. A urine specimen comes to the laboratory 7 hours after it is obtained. It is acceptable for
culture only if the specimen has been stored:
a: at room temperature
d: with a preservative additive - answer✔✔b: at 4-7 degrees Celsius; Storage must inhibit
bacterial growth but not kill the bacteria. For this reason freezing and additives are not
acceptable. The most common method of preservation is refrigeration.
Which of the following would be affected by allowing a urine specimen to remain at room
temperature for 3 hours before analysis?
a: occult blood
b: specific gravity
c: pH
d: protein - answer✔✔c: pH; Consider whether a substance can increase or decrease outside the
body. Blood can' be produced outside of the body and although RBCs can rupture they will still
make a positive result on a biochemical strip. The amount of solutes won't change, so specific
gravity won't change. Protein won't increase or decrease. pH is affected by metabolism of the
urine components by bacteria, and room temperature is warm enough for this to occur.
MLS 9. A 24-hour urine from a man who had no evidence of kidney impairment was sent to the
laboratory for hormone determination. The volume was 600 mL, but there was some question as
to the completeness of the 24-hour collection. The next step would be:
a: perform the hormone determination, since 600 mL is a normal 24 hour urine volume
b: check the creatinine level; if it is <1 g, do the procedure
c: report the hormone determination in mg/dL in case the specimen was complete
d: check the creatinine level; if it is >1 g, do the procedure - answer✔✔d: check the creatinine
level; if it is >1 g, do the procedure. The reader should know that the approximate volume of a
daily void, which is approximately 1500 mL, but can range from 600-2000 mL. In order to
determine if 600 mL is the actual volume, or some sample was missed, evaluate the creatinine.
Creatinine is excreted at approximately 1.2 mg/24 hour. Now evaluate the choices. A) could be
correct but 600 mL is unusually low. B) is incorrect because the creatinine is too low. C) is not
correct because it does not answer the medical question. This leaves D) as the correct answer
since a creatinine level greater than 1 g.
10. Failure to observe RBC casts in a urine specimen can be caused by:
a: staining the specimen
b: centrifuging an unmixed specimen
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