Exam (elaborations)
CMB2001 L01 EXAM QUESTIONS WITH 100% CORRECT ANSWERS
- Course
- Institution
CMB2001 L01 EXAM QUESTIONS WITH 100% CORRECT ANSWERS...
[Show more]
Content preview
CMB2001 L01 EXAM QUESTIONS WITH 100%CORRECT ANSWERS
What is the span of the core/basal region of the eukaryotic promoter? - ANSWER
-30 to +30nt
What elements are in the core region of the eukaryotic promoter? - ANSWER
TATA box
Inr (initiator)
DPE (downstream promoter element)
BRE (TFIIB recognition element)
Describe the TATA box consensus sequence - ANSWER -31 to -26nt,
TATAWAWR
Describe the Inr sequence - ANSWER -2 to +4nt, YYANWYY with an A at +1
What is observed in core promoter CpG islands without obvious TATA and Inr
sequences? - ANSWER Lower transcription rate, initiation at multiple start sites
What is the difference between a distal and proximal regulatory promoter element?
- ANSWER Proximal = within 1kb of the start site
Distal = 1kb or more away from the start site
How do you identify promoter elements via sequence comparison? - ANSWER
Analyse base frequency within many genes to compare and identify a consensus
sequence
How do you identify promoter elements via reporter analysis? - ANSWER Insert a
reporter gene downstream of a regulatory sequence within a plasmid and measure
the expression of the reporter protein.
The impact of mutations and control, induction, timing and location of transcription
can be measured by this.
Describe RNA polymerase I - ANSWER Found in the nucleolus, it transcribes 28S,
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller luzlinkuz. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
72964 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now