Exam (elaborations)
ASCP HT EXAM () QUESTIONS WITH ANSWERS
- Course
- Institution
ASCP HT EXAM () QUESTIONS WITH ANSWERS
[Show more]
Seller
Follow
Content preview
ASCP HT EXAM (2024-2025) QUESTIONS WITHANSWERS
Alcohols
Acetone
Acetic Acid*
Coagulates nuclear proteins but not the cytoplasm
Name Non-additive fixatives
Over stained hematoxylin
Decrease incubation in hematoxylin, increase differentiation,
or cut thinner sections.
What issue is seen in this H&E stain? How can you correct
this issue?
Nuclear bubbling
Inadequate fixation or dehydration, or drying slides in a hot
oven before air drying
What issue is seen in this H&E stain? How can you correct
this issue?
Reddish/brown nuclei
Over oxidized hematoxylin or insufficient bluing
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
What issue is seen in this H&E stain? How can you correct
this issue?
Over staining with eosin
Check the concentration of eosin, decrease time in eosin,
ensure correct timing in dehydrating solution, or checking
section thickness
What issue is seen in this H&E stain? How can you correct
this issue?
Uneven staining
Hematoxylin solution is too low
What issue is seen in this H&E stain? How can you correct
this issue?
Feulgen reaction
What stain is shown in the picture?
Tumor of mesenchymal origin
What is a Sarcoma?
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
Tumor of lymphoid tissue?
What is a Lymphoma?
malignant disease with large numbers of immature white
blood cells
What is Leukemia?
Caner that originates in the plasma cell of the bone marrow.
What is a myeloma?
Recognizes many epitopes from one antigen. The antigen is
injected into the host and then the host serum is selected.
Define polyclonal antibodies
One antibody serviced from a single clone B cell. The antigen
is injected into the host and the host is sacrificed.
Define monoclonal antibodies
, ASCP HT EXAM (2024-2025) QUESTIONS WITH
ANSWERS
B-cells
Hybridoma
Myeloma
What makes up the culture used for monoclonal antibodies?
A primary antibody that is labeled with a fluorochrome.
What is a direct IHC method?
A primary antibody with a secondary antibody labeled with a
fluorochrome
What is an indirect IHC method?
Primary antibody
What is the secondary antibody raised against?
A catalyst for biochemical reaction.
What is an enzyme?
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller jackbundi9071. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
84190 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now