Exam (elaborations)
BACE Exam-Knowledge | Questions And Answers Latest {} A+ Graded | 100% Verified
- Course
- Institution
BACE Exam-Knowledge | Questions And Answers Latest {} A+ Graded | 100% Verified
[Show more]
Content preview
BACE Exam-Knowledge | Questions And Answers Latest {2024- 2025} A+ Graded | 100%Verified
Clinical Trial- Phase 1 - - Tests drug in healthy individuals
- Tests for safety, dosage, and side effects
Clinical Trial- Phase 2 - - Tests on larger group of effected individuals
- Tests for efficacy and side effects
Clinical Trial-Phase 3 - - Tests on new and wider demographic
- Tests for long term effectiveness and comparisons with other medications
Clinical Trial- FDA Approval - Treatment determined effective and safe for public use
Clinical Trial- Phase 4 - - Continues to test for effectiveness and safety
- Can be taken off the market if necessary
Cell Harvesting- Indirect Cell Count - Determine concentration of cells is a liquid culture
DOES NOT tell me if the cells are viable vs nonviable- some cells may be dead
Cell Harvesting- Indirect Cell Count Reagents - - Luria Broth(Food Source)
- Shaking Incubator or shaking water bath (Incubator or Water is for 37 C; Shaking-Oxygen)
- Spectrophotometer (measures absorbance values to give concentration)
Cell Harvesting- Direct Cell Count - Determine concentration of cells in agar place
Cell Harvesting- Direct Cell Count Reagents - - Agar Plate
- Luria Broth(food source)
, - Incubator (37 C)
- Physically Count- the amount the colonies and calculate the concentration
Gram Staining - Determine if peptidoglycan is present in cell wall
Gram Staining Reagents - - Crystal Violet=Gram Positive
- Iodine= fixation
- Alcohol= decolorizer
- Safranin= Gram negative
Inserting into the Cell - Neutralize cell membrane and substance being inserted now passive disuse can
occur
Inserting into the Cell- Reagents - - Cofactors: CaCl2 or MgCl2
- Heat-shock (chemical transformation= prokaryotic) or Electricity/Electroporation (Transfection=
Eukaryotic Cells)
Lysing the Cell - Breaking (destroying cell membrane) open the cell to extract DNA, mRNA, or protein
Lysing the Cell- Reagents - - Detergent: SDS (DNA Extraction) or Lysozyme (Protein Extraction)
- Freeze-Thaw Method: Freezer, water bath/dry bath, vortexer
PCR - Copy DNA
PCR- Reagents - - DNA Sample (denaturing)
- Primers (annealing)
- Nucleotides/dNTPs (elongation)
- Taq Polymerase (elongation)
- Mix Buffer (maintain pH)
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller oneclass. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.48. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
82871 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now