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BACE Practical Review | Questions And Answers Latest {} A+ Graded | 100% Verified
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BACE Practical Review | Questions And Answers Latest {} A+ Graded | 100% Verified
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BACE Practical Review | Questions And Answers Latest {2024- 2025} A+ Graded | 100%Verified
Restriction Enzyme (Restriction Endonuclease) - An enzyme produced chiefly by certain bacteria, having
the property of cleaving/cutting/digesting DNA molecules at or near a specific sequence of bases.
Spectrophotometer - Used to measure protein or DNA concentration; an apparatus for measuring the
intensity of light in a part of the spectrum, especially as transmitted or emitted by particular substances.
Horizontal Gel Electrophoresis Box - Used to run DNA agarose gels.
Vertical Gel Electrophoresis Box - Used to run protein gels (PAGE or polyacrylamide gel electrophoresis).
Buffer - A solvent that is resistant to fluctuations in pH, because it is made with a combination of weak
acid and its conjugate bases.
Acid - A chemical substance that neutralizes alkalis, dissolves some metals, and turns litmus red;
typically, a corrosive or sour-tasting liquid of this kind.
Base - A substance capable of reacting with an acid to form a salt and water, or (more broadly) of
accepting or neutralizing hydrogen ions.
Genomic DNA Digestion - The cleaving of linear DNA; for example- in genomic DNA when three cuts are
made with a restriction enzyme four fragments result.
Plasmid DNA Digestion - The cleaving of circular DNA; for example- in plasmid DNA when three cuts are
made with a restriction enzyme three fragments result.
Kilo- - 10^3
Ex. 1 kiloliter (kL) = 1000 liters (L)
Milli- - 10^-3
, Ex. 1 molar (M) = 1000 mM
Micro- - 10^-6
Ex. 1 gram (g) = 1,000,000 micrograms (ug)
Loading Dye - A solution added to an electrophoresis sample to give it color and density; 1 microliter is
added for every 5 milliliters of sample.
Agarose - A polysaccharide, generally extracted from certain red seaweed, used to make gels for DNA
electrophoresis.
pH Scale - Measures how acidic or basic a substance is; ranges from 0 to 14; logarithmic scale- ex. pH 4
to pH 2.. pH 2 is 100 times more acidic.
Significant Figures - Each of the digits of a number that are used to express it to the required degree of
accuracy, starting from the first nonzero digit; all non zero digits are significant; leading zeros (0.002) are
not significant; captive zeros (1001) are significant; trailing zeros (100 or 100.0) are only significant if a
decimal is present anywhere within the measurement.
Spectrophotometry - Used to determine the amount, or concentration, of a protein (or other substance)
present in a sample by measuring how much it absorbs light as it passes through the sample solution.
Occupational Safety & Health Administration (OSHA) - Government agency that examines workplace
conditions to ensure safety.
Safety Data Sheet (SDS) - Document that provides comprehensive information about a substance or
mixture for use in workplace chemical management.
Personal Protective Equipment (PPE) - Equipment worn to minimize exposure to a variety of hazards.
Independent Variable - Variable that represents inputs or causes and is controlled by the experimenter.
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