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BACE Review Biotechnology 2 | Questions And Answers Latest {} A+ Graded | 100% Verified
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BACE Review Biotechnology 2 | Questions And Answers Latest {} A+ Graded | 100% Verified
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BACE Review Biotechnology 2 | Questions And Answers Latest {2024- 2025} A+ Graded |100% Verified
Structural component of cell membrane, Ex: Phospholipid - Lipid
A chemical bond that involves the sharing of electron pairs between atoms. -
What is the most likely reason there is an unexpected band in a PCR reaction? - Using too low temp.
What should be included on the gel to enable you to determine actual sizes of the DNA fragments? -
Molecular weight marker and DNA Ladder
What is MRNA? - What the order of amino acids on a polypeptide chain is encoded by
Who has final say if a production batch may be released for sale or use? - Function of Quality Assurance
(QA) personnel?
What contributed to biotechnology in the 1950's? - The 3D structure of DNA is revealed.
What is agar? - Solid media on which bacteria grow
What part of an amino acid differs from one to the other? - R group
What is secondary protein structure? - Occurs when amino acid sequence is linked by hydrogen bonds.
What are the properties of the carbon atom that make the diversity of carbon compounds possible? -
Form tetra relevant bonds, highly stable/unstable, able to form short and long chains
A polypeptide is an ___ chain of amino acids - Unbranched
,Are lipids hydrophilic or hydrophobic? - Hydrophobic
Structural component of plant cell walls, Ex: Cellulose - Carbohydrate
Catalyze biochemical reactions, Ex: Enzyme - Protein
Store and transfer genetic information in the cell, Ex: RNA - Nucleic Acid
What is antiparallel DNA? - The nucleotide strands of DNA are oriented in opposite directions in a
structure that is described as
An enzyme that cuts DNA after recognizing a specific sequence of DNA. - Restriction enzyme
Form the phospholipid bilayer of the plasma membrane. - Phospholipid
Identify and neutralize foreign objects such as bacteria and viruses. - Antibody
Rules over cell functions which include cell division, morphing of the shape of the cells, cell mobility and
other contractile properties/Helps in muscle contraction - Actin/Myosin
Why are enzyme solutions always prepared using a buffered solvent at a specific pH? - Enzymes lose
function outside of a narrow pH range
How many amino acids are there? - 20
Why does the rate of a chemical reaction increase with increasing temperature? - Leads to increase in
high energy collisions.
What affects rate of chemical reaction? - Concentration of reactants, heat, the presence of a catalyst
,Converts energy into ATP; take place in mitochondria - Cellular respiration
Cellular energy with oxygen - Aerobic respiration
Cellular energy without oxygen - Anaerobic respiration
What is meiosis and what happens if it does not happen during sex-cell development in plants? - Process
where a single cell divides twice to produce four cells containing half the original amount of genetic
information (sex cells). They go through mitosis.
Denature proteins and give them negative charge - What is the purpose of SDS when used in SDS
electrophoresis?
Whats difference between diploid/haploid/zygote? - Haploid: One set of chromosomes; gametes
Diploid: Two sets of chromosomes; somatic cells
Zygote: 2 haploid gametes fuse during fertilization to form a diploid cell (zygote)
After a mitotic division, how many chromosomes do daughter cells have compared with the parent cell?
- Same as parent; 46
Alternative forms of a gene that arise by mutation and are found at the same place on a chromosome. -
Alleles
Are humans diploid or haploid? - Diploid
Genes encode for proteins. During translation, MRna encodes a blueprint to make the proteins. In
transcription, DNA makes complimentary RNA. - Translation and Transcription
The replication of DNA, in which each original strand of DNA serves as a template for a new strand, is
called: - Semiconservative replication
, What is polymerase chain reaction? - A technique used in molecular biology to amplify a single copy or a
few copies of a piece of DNA across several orders of magnitude
Enzymes involved with in vivo DNA replication, and which are also used as tools in biotechnology
include: - Polymerase and Ligase
DNA does not contain base pair - Uracil
Adenine and guanine - Purines
Thymine and cytosine - Pyrimidines
(PCR) requires what enzyme to be thermostable (resistant to high temperatures) in order to "amplify" a
sample of DNA? - DNA Polymerase
If you think of recombinant DNA technology as cutting and pasting pieces of DNA, which molecule
represents the glue? - Ligase
Bacteria cell DNA is divided into operons. Describe an operon using the terms promoter, operator, and
structural gene. - An operon consists of a group of structural genes that code for enzymes involved in a
metabolic pathway. They are under control of a promoter (a short segment of DNA to which the RNA
polymerase binds to initiate transcription) and regulated by an operator.
Complimentary base pairing holding two DNA strands together is due to: - Hydrogen bonding
What are phospholipids found in? - Cell membranes and endoplasmic reticulums
What are polysaccharide useful for? - Store the energy we gain from consuming food
Lipids are composed mostly of what atoms? - Carbon and hydrogen
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