after successful bacterial transformation with plasmid DNA, you plan to isolate the plasmid DNA from
the genomic DNA using a miniprep. what should you not do? - use water to precipitate and wash DNA
how would you describe a tertiary protein structure? - the three dimensional shape of the polypeptide
chain once is folded
what is true about about protein synthesis? - transfer RNA (tRNA) molecules that carry amino acids
you observe an unknown cell under a microscope. a nucleus is visible. what kind of cell is it? - eukaryotic
under normal physiological conditions, the cell's plasma membrane prevents exogenous nucleic acids
from entering the cell. this is because the membrane is: - impermeable to charged substances
what diffuses from the plant shoot tips to cause cell differentiation? - hormone
what is the gram stain procedure in order? - crystal violet as stain, iodine as mordant, alcohol as
decolorizing agent, gram safranin as counter stain
in the ABO blood type system, what is the universal donor? - type O
describe t-cells - process and present antigens on their cell surfaces
describe b-cells - produce antibodies
describe antibodies - bind and label pathogens
, to start the process of transcription, RNA polymerase binds to the ________________ to initiate mRNA
synthesis - operon
utilizing a patient's own cells to produce a novel drug or medical treatment is called? - personalized
medicine
monoclonal antibodies are derived from the fusion of tumor cells and B cells, which are then grown in
cell culture. what is this technique called? - hybridoma technology
what technique would you use to distinguish a GMO-containing food from a non-GMO-containing food?
- PCR
golden rice is a rice variety genetically engineered to produce beta-carotene, a precursor of vitamin A.
what is not a benefit? - encouraging crop biodiversity
what is an ethical use of genetic screening? - developing personalized medical treatments
prior to testing in humans, a new drug candidate is tested for safety what regulatory practices? - cGLPs
what is correct in regards to clinical trials? - the emphasis of a Phase I clinical trial is on treatment safety
When a co-worker wears nitrile groves out of the lab, what is the appropriate action? - notify the co-
worker to not wear gloves outside the lab
you notice a tear in the packaging of a disposable loop that you are about to use for an experiment.
what is the correct response? - discard. the content and packaging is compromised and the risk of
contamination is high
what is a technique for harvesting bacteria from overnight liquid culture? - streaking
when is it acceptable to aseptically transfer bacteria without wearing gloves? - it is never acceptable
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller oneclass. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.48. You're not tied to anything after your purchase.