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Biosafety Levels Exam | Questions And Answers Latest {} A+ Graded | 100% Verified
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Biosafety Levels Exam | Questions And Answers Latest {} A+ Graded | 100% Verified
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Biosafety Levels Exam | Questions And Answers Latest {2024- 2025} A+ Graded | 100%Verified
BSL 1 - microorganisms not known to cause disease in healthy adults; standard microbiological practices
are followed, PPE is not required, a sink must be available for hand washing; examples are bacillus
subtilis, serratia marcescens, staphylococcus saprophyticus
BSL 2 - indigenous microorganisms that can lead to diseases of varying severity in healthy adults; BSL 1
procedures plus lab access is restricted, PPE must be worn including lab coats, gloves, and eye
protection, work that may generate aerosols or splashes are performed in a biological safety cabinet,
contaminated waste is autoclaved, the lab has self closing doors, a sink and eye wash are available;
examples: s. aureus, streptococcus pyogenes, and salmonella
BSL 3 - indigenous or exotic microorganisms that cause serious or potentially lethal disease through
respiratory transmission; BSL 2 procedures plus medical surveillance of lab employees, immunizations
may be required; locking, double door access separates the labs work area from the other part of the
facility, PPL must be worn including lab coats, gloves, eye protection, and respirators; all work is
performed in biosafety cabinet, decontamination of all waste and lab clothing before laundering; lab
must have negative air flow, exhaust air cannot be recirculated; hands free sink and eye wash must be
available; examples: mycobacterium tuberculosis, bacillus anthracis, francisella tularensis
BSL 4 - microorganisms that are dangerous and exotic with high risk of aerosol transmitted infections;
rarely are there treatments or vaccines for these microorganisms, and the diseases they cause are
frequently fatal; BSL 3 procedures plus changing clothes before entering lab, shower upon exiting lab,
decontaminate all materials before exiting lab, all work is done in class 3 biosafety cabinet or by wearing
a full body, positive pressure suit, lab must have negative air flow, and exhaust air cannot be
recirculated, the lab must be in a separate building or in an isolated and restricted zone of the building
fungi, 1 - morphology & biosafety level of aspergillus
positive rod, 1 - gram stain, morphology & biosafety level of bacillus cereus
positive rod, 1 - gram stain, morphology & biosafety level of bacillus megaterium
positive rod, 1 - gram stain, morphology & biosafety level of bacillus subtilis
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