Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - answerNitrogen
Which of the proteins is soluble in water? - answerAlbumin
How are amino acids joined together to form a chainlike structure? - answerPeptide
bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or
chemical treatment. What does denaturation of proteins refer to? - answerAlteration of
tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - answerCystinuria
These aminoacidopathies are caused by an enzyme deficiency: - answerMaple syrup
disease, phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - answerFive
Which reagent is employed in the serum protein determination? - answerBiuret
Why should hemolyzed serum not be used for total protein analysis? -
answerHemoglobin is absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - answerIt is relatively free
from interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - answerFollows Beer's law for a
reasonable range of protein, depends on the presence of at least two peptide linkages,
and is insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
answerThere is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - answerAlbumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
, Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - answer3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - answerActive cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10%
beta-globulin, and 45% gamma globulin, then what condition may exist in the patient? -
answerMonoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? -
answerCarbohydrates
All of the following are glycoproteins: - answerCeruloplasmin, follicle-stimulating
hormone (FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - answerCryoglobulin
Which one of the following applies to cryoglobulins? - answerThey are temperature-
sensitive proteins
Which of the following statements is true of albumin? - answerIt is produced in the liver
and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - answerDown
syndrome
Which one of the following proteins transports iron? - answerTransferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - answerBlood urea
nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? -
answerAmmonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxomine method? - answerThe method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values?
- answerElevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - answerJaffe
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