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(MB) ASCP Practice Exam Questions and Complete Solutions Graded A+

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  • ASCP CG
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  • ASCP CG

(MB) ASCP Practice Exam Questions and Complete Solutions Graded A+

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  • August 22, 2024
  • 35
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • ASCP CG
  • ASCP CG
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(MB) ASCP Practice
Exam Questions
and Complete
Solutions Graded
A+
Denning [Date] [Course title]

,Which of the following is not a component of a nucleotide?

Phosphate group

Anti-codon

Ribose sugar

Nitrogen base - Answer: Anti-codon



According to Chargaff's rule of base pairing, adenine pairs with: - Answer: Thymine



What genes would be screened in a breast cancer panel? - Answer: HER2, ERBB2, BRCA1



Next Generation Sequencing uses: - Answer: Short sequence reads



Purines and pyrimidines differ from each other in that: - Answer: Purines have two rings; pyrimidines
have one ring



The purines are:

Cytosine and uracil

Adenine and thymine

Thymine and cytosine

Adenine and guanine - Answer: Adenine and guanine



What is the rate of mutation per round of DNA replication?

1 in 1,000 base pairs

1 in 10,000 base pairs

1 in 1,000,000 base pairs

1 in 1,000,000,000 base pairs - Answer: 1 in 1,000,000,000 base pairs



The rate of DNA migration through an agarose gel during electrophoresis does not depend on which of
the following factors?

,Net charge of the molecule

Size of the molecule

Shape of the molecule

Nucleotide sequence of the molecule - Answer: Nucleotide sequence of the molecule



What are the phases in a qPCR Amplification Plot?

Initiation, exponential, plateau

Baseline, exponential, plateau

Baseline, threshold, exponential, plateau

Baseline, initiation, threshold, exponential, plateau - Answer: Initiation, exponential, plateau



Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?



5'-GAC

3'-GAC

3'-CTG

5'-GTC - Answer: 3'-GAC



A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntington gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?

25 CAG repeats in the Huntington gene

85 CAG repeats in the Huntington gene

25 CGA repeats in the Huntington gene

85 CGA repeats in the Huntington gene - Answer: 85 CAG repeats in the Huntington gene



Which two HPV types are responsible for most cases of cervical cancer?

, 16 and 18

31 and 59

16 and 58

44 and 59 - Answer: 16 and 18



Replication forks, known as origins of DNA replication, are created by this enzyme:

Ligase

Taq Polymerase

Primase

Helicase - Answer: Helicase



Mutation in what gene is associated with Fragile X syndrome? - Answer: FMR1



Mantle cell lymphoma (MCL) is caused by what translocation? - Answer: t(11;14)



This polymerase is involved in "initiation of DNA replication and has primase activity": - Answer: Pol α



Its discovery shed light on why there is simultaneous, though not continuous, synthesis of DNA on both
leading and lagging strands of DNA:

Klenow fragment of DNA polymerase

Okazaki fragments

Sanger fragments

RNA fragments - Answer: Okazaki fragments



What gene is measured following treatment with imatinib (Gleevec)?

FLT3

BCR/Abl

Jak2

MAPK - Answer: BCR/Abl

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