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BTECH Review Exam 2 Questions & Answers 2024/2025 $8.49   Add to cart

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BTECH Review Exam 2 Questions & Answers 2024/2025

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BTECH Review Exam 2 Questions & Answers 2024/2025 what applies to bacteriophages? - ANSWERSBacteriophages recognize molecules on bacterial surface to infect All phages use the hosts replication machinery to some extent Most phages have narrow host range Bacteriophage genomes can be R...

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  • August 22, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • BTECH
  • BTECH
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BTECH Review Exam 2 Questions &
Answers 2024/2025

what applies to bacteriophages? - ANSWERSBacteriophages recognize molecules on bacterial surface to
infect



All phages use the hosts replication machinery to some extent



Most phages have narrow host range



Bacteriophage genomes can be RNA, but mostly DNA



In vitro transcription of the SARS-2 spike gene makes use of the __________ - ANSWERST7 gp10
promoter



The antirestriction protein of T7: - ANSWERSprotects T7 DNA from bacterial endonucleases



What is the enzymatic function of gene product 1 in T7? - ANSWERSmRNA synthesis



Which phage-encoded protein catalyzes the formation of phosphodiester bonds between nucleotides? -
ANSWERSDNA ligase



In the pET system for recombinant protein expression, in the absence of IPTG: - ANSWERSBinding of T7
RNA polymerase to the gp10 promoter is obstructed



Which gene or feature is employed in the pET system? - ANSWERST7 gp10 promoter



LacI

, T7 RNA polymerase



plac



What is a major advantage of bacteriophages as antimicrobial agents over small molecule antibiotics? -
ANSWERSless killing of friendly human bacteria



What is a pathogenic bacterial genus for which a bacteriophage is being developed for treatment, and is
commonly implicated in bacterial food poisoning? - ANSWERSlisteria

escherichia

salmonella



What applies more to generalized than to specialized transduction? - ANSWERSLacks site specificity for
integration in the bacterial chromosome



specialized transduction - ANSWERSDNA of the host bacterium tends to get completely degraded



Preferentially transfers only a certain region of the bacterial chromosome



Mostly bacterial sequences close to attB site are transferred during transduction



The transducing phage tends to have a very narrow host range



Which event in bacteriophage lambda will favor lysis over lysogeny? - ANSWERSIncreased expression of
the cro repressor



Which event in bacteriophage lambda will favor lysogeny? - ANSWERSAntitermination mediated by T
protein



Autocleavage of CRO dimers upon contact with RecA-ssDNA nucleoprotein

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