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secuenciación de genes y genomas

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presentación del curso de ingeniería genetica , tema secuenciación de genes y genoma

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  • August 23, 2024
  • 38
  • 2024/2025
  • Presentation
  • Unknown
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SECUENCIACIÓN
DE GENES Y
GENOMAS

, INTRODUCCIÓN


► Secuenciación: Método mediante el cual puede conocerse el
orden preciso de los nucleótidos de un fragmento de DNA


► Inicialmente estas técnicas fueron aplicadas a genes
individuales, desde principios de 1990 se han obtenido
secuencias de genomas enteros

,Metodología para la secuenciación
de DNA
► Método de terminación de la cadena: Facilidad de
automatización, disminución del tiempo de secuenciación;
eficiencia de hasta 96 secuencias simultáneamente


► Pirosecuenciación o síntesis : Cientos de miles de secuencias
cortas generadas al mismo tiempo

, Secuenciación de DNA por método de
terminación de la cadena

Electroforesis
en gel de
poliacrilamida


Separación
de cadenas
sencillas que
difieren en un
único
nucleótido

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