What is the difference between a nucleoside and nucleotide? - ANS A nucleoside is formed from
the covalent bonding of a base (purine or pyrimidine) to either ribose (RNA) or 2-deoxyribose
(DNA). Nucleotides are built from a nitrogen-containing organic base, a monosaccharide, and
phosphate.
What characteristic makes purines and pyrimidine heterocyclic? - ANS Each has a ring with
nitrogen in the ring instead of all carbon atoms in the ring.
Is this molecule ribose or 2-deoxyribose? Briefly explain what structural information was used to
determine this. - ANS Ribose. This molecule contains an -OH on carbon 2. 2-deoxyribose has
an -H atom on the second carbon.
Is the following a pyrimidine or purine? Provide the name and one-letter symbol. - ANS
Pyrimidine. It is thymine (T).
What is the difference between thymine and uracil? - ANS These two bases differ by a methyl
group. Thymine has an extra methyl group on the ring.
From the course and module information, where in a eukaryotic or prokaryotic cell, is the DNA
located? How about the RNA? - ANS DNA is located in the nucleus of a eukaryotic cell or the
nucleoid of a prokaryotic cell. RNA is found in the nucleus, on the ribosome, and in other parts
of the cell.
Which DNA and RNA bases contain a carbonyl group? - ANS Guanine, cytosine, thymine, and
uracil all contain C=O group attached to the rings. Adenine is the only base that does not.
What constitutes the backbone of DNA? - ANS In DNA, this consists of the alternating
deoxyribose and phosphate groups linked together through covalent bonds.
How many hydrogen bonds form between A and T? - ANS Two hydrogen bonds are formed
between these two bases. G and C form three H-bonds.
Which nucleic acid is single-stranded? - ANS RNA. RNA forms single strands. (Particularly, this
was noted in this module for tRNA.) DNA forms a double helix.
What interactions stabilize the DNA double helix? - ANS Hydrogen bonds stabilize the helix.
Each AT pair and form two hydrogen bonds, and each GC pair forms three hydrogens that
stabilize the helix, permitting it to maintain this shape.
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