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Uta nursing microbiology lab 2 | Questions And Answers Latest {} A+ Graded | 100% Verified
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Uta nursing microbiology lab 2 | Questions And Answers Latest {} A+ Graded | 100% Verified
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Uta nursing microbiology lab 2 | Questions And Answers Latest {2024- 2025} A+ Graded |100% Verified
factors that influence biosafety levels - infectivity, severity of disease, transmissibility, and nature of
work conducted in the lab.
microbio labs at uta are considered - bsl-2
level 1 description - Microbes in BSL-1 labs are not known to consistently cause disease in healthy adults
and present minimal potential hazard to lab workers and the environment. An example of a microbe
that is typically worked with at a BSL-1 is a nonpathogenic strain of Escherichia coli.
level 2 description - Microbes in BSL-2 labs pose moderate hazards to lab workers and the environment.
The microbes are typically indigenous and associated with diseases of varying severity. An example of a
microbe that is typically worked with at a BSL-2 laboratory is Staphylococcus aureus.
level 3 description - Microbes in BSL-3 labs can be either indigenous or exotic, and they can cause
serious or potentially lethal disease through respiratory transmission. Respiratory transmission is the
inhalation route of exposure. One example of a microbe that is typically worked with in a BSL-3
laboratory is Mycobacterium tuberculosis, the bacteria that causes tuberculosis.
level 4 description - Microbes in BSL-4 labs are dangerous and exotic, posing a high risk of aerosol-
transmitted infections. Infections caused by these microbes are frequently fatal and without treatment
or vaccines. Two examples of microbes worked with in a BSL-4 laboratory include Ebola and Marburg
viruses.
precautions of level 1 - Lab work is performed at a bench
Personal Protective Equipment (PPE) includes lab coat/apron, goggles, and gloves worn as needed
Sink is available for hand washing
Lab room should be separated with a door from the rest of the building
precautions for level 2 - All requirements for BSL-1 plus:
Access to these labs is restricted to approved personnel only
, Labs have self closing doors
Eye wash station(s) are available
An autoclave or equal method of sterilization is available
precautions for level 3 - All requirements for BSL-2 plus:
Personnel must have necessary vaccinations
Work is perform in a biohazard safety cabinet
Air supply to the lab room must be drawn from a clean supply and cannot be recirculated
Lab room must have two self closing and lockable doors
precautions for level 4 - All requirements for BSL-3 plus:
Personnel must change clothing and shower before and after entering these labs.
Biosafety cabinets must be a special level III cabinet.
The laboratory is located in a separate building or in an isolated and restricted zone of the building.
The laboratory has dedicated supply and exhaust air, as well as vacuum lines and decontamination
systems.
aseptic technique - involves a combination of protocols that collectively maintain sterility, or asepsis, to
prevent contamination of a patient or surface with microbes and infectious agents.
Critical to prevent contamination of sterile surfaces (on tissue or non-biological surfaces).
media - medium
growth medium - substance in which microorganisms are encouraged to grow
provide specific nutrients to allow microbiologists to observe certain characteristics of the microbes
they are interested in.
agar - mixture of polysaccharide and pectin derived from red algae (phylum Rhodophyta).
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