Unit 4 - Genetic information, variation and relationships between organisms
Summary
Summary PCR and cloning notes
16 views 0 purchase
Course
Unit 4 - Genetic information, variation and relationships between organisms
Institution
AQA
These notes helped me get into medical school, I completed my a levels at Guildford high school which consistently ranks among the top schools in the UK and is known for their academic rigour. I made these notes as a summary of the power points that we were taught off of.
Unit 4 - Genetic information, variation and relationships between organisms
All documents for this subject (131)
Seller
Follow
anastazjaohearn
Content preview
resistance to
> MRNA is easier to find desired gene. reverse gene cloning ↓
A
& J
asmid I
plrecognition
desire set
g
no introns transcriptase makes (copies) CDNA
. Restriction Invitro (PCR) in vivo
·
endonucleases cut up CDNA
making sticky ends
. separate DNA95% plasmids (vector)
g
resistance
fragments to plasmids
. removed with To test transfer,
DNA
ligase joins up annealing primers gene a to B
%
55 C restriction will be resistant to
B
(sticking) enzyme
gene machine shows base sequence based ·
tag polymerase ·
join Vector and DNA and not to A
*
<
g
on a protein PCR
C an
. be used to amplify adds nucleotides 72%
fragment with DNA ligase
Interrupted
↑ >
-
fast ·
Recombinant DNA introduced
to bacterium
Selectable
markers
genetic
a
universal
gene therapy
>
-
non-overlaping
& degenerate
Tag
polymerase
↓
will not
denature
at 95%
-
genetic fingerprinting
extraction from sample
digestion < restriction endonucleases
separating -
gel electrophoresis
*
Annealing
separation n transfered to nylon membrane
risk of contaminated DNA
amplifying a hybridisation< radioactive probes attach
·
development placed onto
xray film
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller anastazjaohearn. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $3.99. You're not tied to anything after your purchase.
