Liquid Samples - ANSBlood, spleen, bone marrow, fresh marine water
Solid Tissue Samples - ANSTumors, organs, plants
Cons of enzymatic disruption methods - ANSCan strip surface epitopes
How to prevent coagulation - ANSACD, EDTA, heparin, or physically disrupt cells using a
method such as vortexing
How long can whole blood remain at room temperature? - ANS24 hours, then refrigerate for up
to 72 hours
Cells that are too large for flow cytometry - ANSCardiomyocytes and Megakaryocytes
Factors to consider when fixing to preserve the epitope - ANSFixative type, fixation time,
temperature, fluorochrome compatibility, surface marker compatibility
Alcohol fixation cannot be used on these fluorochromes - ANSPE and GFP
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