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ASCP HT EXAM QUESTIONS WITH CORRECT ANSWERS!!
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ASCP HT EXAM QUESTIONS WITH CORRECT ANSWERS!!
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Name: Score:613 Multiple choice questions
Term 1 of 613
The oxidized hematoxylin product
Clearance Angle
What reagents make Bouin
Define: Hematein
Dioxane can be used for what purpose
Definition 2 of 613
Can you prevent mercuric chloride pigment?
Adenocarcinomas
No
Fontana-Masson stain
PAS-H
,Definition 3 of 613
What are the reagents used in the Mallory PTAH procedure?
Acid mucopolysaccharides & sialomucins - Deep Blue
Nuclei - Pink to Red
Cytoplasm - Pink
Reducing substances - Blue/Green
Goblet cells and mucin - Rose
Background - Yellow/Green
Capillary basement membranes, fungi, corpora amylacea, and senile plaque - Rose
Myelin sheath - Blue to Blue-Green
Nuclei - Purple
PTAH solution (Hematoxylin, phosphotungstic acid, and DI water), Gram iodine (Iodine,
potassium iodide, DI water), 5% Sodium thiosulfate, 0.25% Potassium permanganate, 5%
Oxalic acid.
Definition 4 of 613
Define cause of microvibrations (microchatter)
Over dehydration
Dull blade
Too much blade tilt
Cutting too rapidly
Used for better preservation of carbohydrates such as glycogen
Nuclei and hemofuchsin - Bright red
Hemosiderin - Blue
Background - Pink
When dehydration and clearing can destroy the tissue or cells of interest
,Definition 5 of 613
What reagents are used in the Churukian-Schenk method?
Chromic acid 5%, Schiff reagent, Sulfurous acid rinse, and Fast green 1:5000
Citric acid 0.3%, Acidified water, Silver nitrate 0.5%, reducing solution (Sodium sulfite
anhydrous, Hydroquinone, and DI water), and nuclear fast red.
Oil Red O Solution ( Oil Red O, 98% Isopropyl, DI water),
Harris Hematoxylin
Fixative reagent (Fast Green Dye in Methanol)
Solution I (Xanthene dye - usually eosin G)
Solution II (Thiazine dye - usually methylene blue and azure A)
Definition 6 of 613
What are some characteristics of chelating decalcification?
Tissues must be very small or will take weeks to decal
Used on bone that requires IHC or enzyme staining
Acts very slowly but preserves all cellular components
Requires frequent changes of solution
PTAH solution (Hematoxylin, phosphotungstic acid, and DI water), Gram iodine (Iodine,
potassium iodide, DI water), 5% Sodium thiosulfate, 0.25% Potassium permanganate, 5%
Oxalic acid.
Spirochetes, H.Pylori, L pneumophila, and other nonfilamentous bacteria - Dark brown to
Black
Background - Light Yellow
Weights iron hematoxylin, 0.25% Metanil yellow (Metanil yellow, Acetic acid, DI water),
Mucicarmine solution (Carmen alum lake, Aluminum hydroxide, ethyl alcohol, aluminum
chloride anhydrous, and DI water)
, Definition 7 of 613
What thickness should tissue be sectioned for the Bielschowsky method?
8-10um
Crystal Violet
6-8um
Agar & Gelatin
Definition 8 of 613
What are the color results of the Gomori 1 Step Trichrome procedure?
Nuclei - Blue
Cytoplasm of leukocytes - Maybe shades of pink, blue, or gray depending on cell type and
development
Bacteria - Blue
Nuclei - Black
Cytoplasm, keratin, and muscle fibers - Red
Collagen and mucin - Green or Blue (Depending on connective tissue stain)
Elastic fibers - Blue to Black
Nuclei - Blue to Black
Collagen - Red
Other tissue elements - Yellow
Luxol fast blue 0.1%, Lithium carbonate 0.05%, schiffs solution, Hematoxylin, and Periodic
acid 0.5%
Definition 9 of 613
At what thickness should sections be cut for the luxol fast blue method?
Cardiac
10-15um
Agar & Gelatin
Skeletal
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