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ASCP HT EXAM QUESTIONS WITH CORRECT ANSWERS!!

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ASCP HT EXAM QUESTIONS WITH CORRECT ANSWERS!!

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  • August 26, 2024
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613 Multiple choice questions

Term 1 of 613
The oxidized hematoxylin product

Clearance Angle

What reagents make Bouin

Define: Hematein

Dioxane can be used for what purpose

Definition 2 of 613
Can you prevent mercuric chloride pigment?

Adenocarcinomas

No

Fontana-Masson stain

PAS-H

,Definition 3 of 613
What are the reagents used in the Mallory PTAH procedure?

Acid mucopolysaccharides & sialomucins - Deep Blue
Nuclei - Pink to Red
Cytoplasm - Pink


Reducing substances - Blue/Green
Goblet cells and mucin - Rose
Background - Yellow/Green

Capillary basement membranes, fungi, corpora amylacea, and senile plaque - Rose
Myelin sheath - Blue to Blue-Green
Nuclei - Purple

PTAH solution (Hematoxylin, phosphotungstic acid, and DI water), Gram iodine (Iodine,
potassium iodide, DI water), 5% Sodium thiosulfate, 0.25% Potassium permanganate, 5%
Oxalic acid.

Definition 4 of 613
Define cause of microvibrations (microchatter)

Over dehydration
Dull blade
Too much blade tilt
Cutting too rapidly

Used for better preservation of carbohydrates such as glycogen


Nuclei and hemofuchsin - Bright red
Hemosiderin - Blue
Background - Pink

When dehydration and clearing can destroy the tissue or cells of interest

,Definition 5 of 613
What reagents are used in the Churukian-Schenk method?

Chromic acid 5%, Schiff reagent, Sulfurous acid rinse, and Fast green 1:5000

Citric acid 0.3%, Acidified water, Silver nitrate 0.5%, reducing solution (Sodium sulfite
anhydrous, Hydroquinone, and DI water), and nuclear fast red.


Oil Red O Solution ( Oil Red O, 98% Isopropyl, DI water),
Harris Hematoxylin

Fixative reagent (Fast Green Dye in Methanol)
Solution I (Xanthene dye - usually eosin G)
Solution II (Thiazine dye - usually methylene blue and azure A)

Definition 6 of 613
What are some characteristics of chelating decalcification?

Tissues must be very small or will take weeks to decal
Used on bone that requires IHC or enzyme staining
Acts very slowly but preserves all cellular components
Requires frequent changes of solution

PTAH solution (Hematoxylin, phosphotungstic acid, and DI water), Gram iodine (Iodine,
potassium iodide, DI water), 5% Sodium thiosulfate, 0.25% Potassium permanganate, 5%
Oxalic acid.

Spirochetes, H.Pylori, L pneumophila, and other nonfilamentous bacteria - Dark brown to
Black
Background - Light Yellow

Weights iron hematoxylin, 0.25% Metanil yellow (Metanil yellow, Acetic acid, DI water),
Mucicarmine solution (Carmen alum lake, Aluminum hydroxide, ethyl alcohol, aluminum
chloride anhydrous, and DI water)

, Definition 7 of 613
What thickness should tissue be sectioned for the Bielschowsky method?

8-10um

Crystal Violet

6-8um


Agar & Gelatin

Definition 8 of 613
What are the color results of the Gomori 1 Step Trichrome procedure?

Nuclei - Blue
Cytoplasm of leukocytes - Maybe shades of pink, blue, or gray depending on cell type and
development
Bacteria - Blue

Nuclei - Black
Cytoplasm, keratin, and muscle fibers - Red
Collagen and mucin - Green or Blue (Depending on connective tissue stain)

Elastic fibers - Blue to Black
Nuclei - Blue to Black
Collagen - Red
Other tissue elements - Yellow

Luxol fast blue 0.1%, Lithium carbonate 0.05%, schiffs solution, Hematoxylin, and Periodic
acid 0.5%

Definition 9 of 613
At what thickness should sections be cut for the luxol fast blue method?

Cardiac

10-15um

Agar & Gelatin

Skeletal

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