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Straighterline Microbiology Lab BIO250L Lab 4 Selective Media & Agar Worksheet (2024/2025) Scored 100%
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Straighterline Microbiology Lab BIO250L Lab 4 Selective Media & Agar Worksheet () Scored 100%
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StraighterlineMicrobiology Lab
BIO250L Lab 4 Selective
Media & Agar
Worksheet
(2024/2025)
Scored 100%
, BIO250L
Student Name:
Access Code (located on the lid of your lab kit):
Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this worksheet. Submissions
without proper formatting, all required photos or sufficient responses will be rejected.
Pre-lab Questions
1. In this lab, you will conduct three different experiments that each cover the topic of selective media and
agar. How are these experiments different from one another? Explain how the different media in each
experiment are utilized in the context of selective vs differential media. The precise goals and kinds of
microorganisms each experiment aims to isolate vary. The selection of media is based on their capacity to
promote the development of particular bacteria (selective growth) or facilitate the differentiation of
bacterial traits (differential expansion). Each experiment's media selection is based on the specific
characteristics of the target bacterium. They have a clear emphasis on identifying and isolating
microorganisms with particular characteristics. Starch agar is used in the bioprospecting experiment to
promote the development of bacteria that specifically break down starch. In Experiment 2, Gram-positive
bacteria are isolated and distinguished according to mannitol fermentation and hemolysis patterns using
Mannitol Salt Agar and Columbia CNA Agar. In Experiment 3, Gram-negative bacteria are isolated and
distinguished using MacConkey Agar and Eosin Methylene Blue Agar as selective media. The classification
process is based on lactose fermentation and colony appearance. To further our understanding of the
diversity and features of microbes, every experiment uses a combination of selective and differential
media specific to the target microorganisms.
2. What is the difference between chemically defined and chemically complex media? Give an example (a
clinical or environmental research application) of when you would use each type of
medium to culture different microorganisms.
Chemically defined media are distinguished from chemically complicated media by their
chemical composition. Chemically defined media, like glucose broth, have well-established
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Lab 4 Selective Media & Agar BIO250L
designs. Conversely, chemically complicated media, like wholesome broth, might vary
from batch to batch and have unknown ingredients.
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