UConn MCB 2610 Final Lab Practical Exam Questions And Correct Answers.
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Course
MCB 2610
Institution
MCB 2610
mear of the culture, you heat fix the slide before air drying it
completely. - answerFALSE
True/False: wiping down the bench with wescodyne is the last thing you do when leaving the
lab. - answerFALSE
What is the magnification value of the ONLY objective lens that should come in contact with
i...
UConn MCB 2610 Final Lab Practical Exam
Questions And Correct Answers.
The ________ method is a quick and easy way to isolate bacteria. - answer✔✔Streak plate
How many times do you get culture from the tube while performing a t-streak? - answer✔✔Once
Who do you contact to reschedule a lab? - answer✔✔Gino
The culture tubes are disposed of in the gray trash bin. True/false? - answer✔✔FALSE
What is the first thing you do after a spill? - answer✔✔Tell the TA
Which of the following is an indirect mode of transmission - an insect, a cellphone, a sneeze? -
answer✔✔Cellphone
What is the last thing you do before leaving lab? - answer✔✔Wash hands for 20 seconds
The outer cone is the hottest part of the flame. True/false? - answer✔✔FALSE
Where are slides disposed of? - answer✔✔Sharps container
What must you do to the loop before every use? - answer✔✔Heat in Bunsen-burner flame
True/False: When making a smear of the culture, you heat fix the slide before air drying it
completely. - answer✔✔FALSE
True/False: wiping down the bench with wescodyne is the last thing you do when leaving the
lab. - answer✔✔FALSE
What is the magnification value of the ONLY objective lens that should come in contact with
immersion oil? - answer✔✔100x
Pertaining to Gram stain, cells that retain crystal violet and appear purple are said to be_____. -
answer✔✔Gram positive
True/False: your name, the date, and sample information goes on the lid of the agar dish. -
answer✔✔FALSE
True/False: heat used during staining causes sporulation. - answer✔✔FALSE
True/False: malachite green stains the vegetative cells green, while the safranin stains
endospores pink. - answer✔✔FALSE
True/False: a vegetative cell is formed in response to certain environmental conditions such as
depletion of nutrients. - answer✔✔TRUE
True/False: Gram stains and spore stains are examples of simple stains. - answer✔✔FALSE
What causes bacterial endospore formation? - answer✔✔Response to environmental conditions,
i.e., lack of nutrients
What are the 4 phases of microbial growth? - answer✔✔Lag, exponential/log, stationary, death
True/False: we will measure the optical density over time to determine growth rate. -
answer✔✔TRUE
Define generation time. - answer✔✔Time for population to double
What does it mean for a culture to be in stationary phase? - answer✔✔Rate of replication
matches rate of death
True/False: you can use oil with the 40x objective lens. - answer✔✔FALSE
True/False: generation time is calculated in the log phase. - answer✔✔TRUE
What does it mean for a culture to be in log phase? - answer✔✔Rapid growth and replication;
each cell doubles
True/False: Gram positive bacteria have a thick peptidoglycan layer which retains safranin and
appears pink. - answer✔✔FALSE
True/False: the purpose of heating during the spore stain is to create an endospore. -
answer✔✔FALSE
What group of organisms does MacConkey agar select against? - answer✔✔Gram positive
True/False: complex media is made with a precise chemical composition. - answer✔✔FALSE
What organism does SF broth select for? - answer✔✔Enterococcus faecalis
What type of bacteria can be differentiated using an EnteroPluri tube? - answer✔✔Enteric
family of bacteria encompassing gram negative, non-sporeforming, facultative anaerobic rods
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