What 4 things must always be done before leaving lab (in order)? - answer✔✔1) Dispose of all
cultures and plates in the appropriate containers
2) Clean your bench with Wescodyne
3) Take off and put away your lab coat
4) Wash your hands for at least 20 seconds with soap and water
Where are plates disposed of? - answer✔✔Gray garbage cans by the door
Where are test tubes and caps disposed of? - answer✔✔Waste buckets under sink
Where are slides and toothpicks disposed of? - answer✔✔Sharps
What first action should be taken for every spill? - answer✔✔Tell a TA
The ___ method is a quick and easy way to isolate bacteria. - answer✔✔T-streak
How many times do you get culture from the tube while performing a t-streak? - answer✔✔1
Who do you contact to reschedule a lab? - answer✔✔Dr. Intrieri
The culture tubes are disposed of in the gray trash bins. (TRUE/FALSE) - answer✔✔False
What is the first thing you do after a spill? - answer✔✔Tell TA
Which of the following is an indirect mode of transmission: an insect, a cellphone, or a sneeze? -
answer✔✔cellphone
What is the last thing you do before leaving lab? - answer✔✔Wash hands
Where are petri dishes disposed of? - answer✔✔Grey binds
Where are slides disposed of? - answer✔✔Sharps
What must you do to the loop before every use - answer✔✔Sterilize/flame
When making a smear of the culture, you heat fix the slide before air drying it completely.
(TRUE/FALSE) - answer✔✔False
Wiping down the bench with Wescodyne is the last thing you do when leaving the lab.
(TRUE/FALSE) - answer✔✔False
What is the magnification value of the ONLY objective lens that should come into contact with
immersion oil? - answer✔✔100x
Pertaining to the Gram Stain, cells that retain crystal violet and appear purple are said to be Gram
____. - answer✔✔Positive
Your name, the date, and the sample information goes on the lid of the agar dish.
(TRUE/FALSE) - answer✔✔False
Pertaining to the Gram Stain, cells that do not retain crystal violet and appear pink are said to be
Gram ___. - answer✔✔negative
What is the purpose of soaking the slide in iodine? - answer✔✔Mordant for crystal violet
The first thing you do before leaving lab is dispose of all culture tubes. (TRUE/FALSE) -
answer✔✔True
Why do you inoculate plates and tubes near the Bunsen burner? - answer✔✔prevent airborne
contamination
Gram stains and spore stains are examples of simple stains. (TRUE/FALSE) - answer✔✔False
What causes bacterial endospore formation? - answer✔✔Nutrient exhaustion
What are the 4 phases of microbial growth? - answer✔✔lag, log, stationary, death
We will measure the optical density over time to determine the growth rate. (TRUE/FALSE) -
answer✔✔True
Define generation time - answer✔✔the amount of time it takes for the population to double
What does it mean for a culture to be in stationary phase? - answer✔✔rate of growth and death
are equal
The purpose of heating during the spore stain is to create an endospore. (TRUE/FALSE) -
answer✔✔False
Generation time is calculated in the log phase. (TRUE/FALSE) - answer✔✔True
You can use oil with the 40x objective lens. (TRUE/FALSE) - answer✔✔False
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