BIO 1404 Practical Test 2|Questions & 100% Correct Answers
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Course
Bio 1404
Institution
Bio 1404
Which one of the solutions is the positive control, and which one of the solutions is the negative control
for the Iodine reaction? - The positive control control is the 1% starch solution and the negative
control is the distilled water.
Biuret Test - Protein ; Peptide Bonds
A VIOLET color is a...
BIO 1404 Practical Test 2|Questions & 100%
Correct Answers
Which one of the solutions is the positive control, and which one of the solutions is the negative control
for the Iodine reaction? - ✔✔The positive control control is the 1% starch solution and the negative
control is the distilled water.
Biuret Test - ✔✔Protein ; Peptide Bonds
A VIOLET color is a positive (+) test for the presence of a protein
A clear BLUE is negative (-) test
The intensity of color relates to the number of bond that react
,Only long-chain polypeptides (with many peptide bonds) produce positive reaction
Which one of the solutions is the positive control, and which one of the solutions is the negative control
for the Biuret reaction? - ✔✔The positive control protein solution and the negative is distilled water.
Do free amino acids have peptide bonds? - ✔✔No, a CU2+ must complex with four to sic
peptide bonds to produce color; therefore, individual amino acids do not react positively
Sudan IV - ✔✔Lipids
Clear; not completely orange is negative
Fully ORANGE (bit foggy) is positive
Based on lipid's solubility in polar solutions or ability to selectively absorb pigments in fat=soluble dyes
Which one of the solutions is the positive control, and which one of the solutions is the negative control
for the Sudan IV reaction? - ✔✔The positive control is the lipid solution and the negative control is
the distilled water.
What do you conclude about the solubility of lipids in polar solvents such as water compared to
nonpolar solvents such as acetone? - ✔✔The acetone had a higher solubility than the water
Dish diphenylamine Test - ✔✔DNA
The intensity of the BLUE color is proportional to the concentration of DNA in sample
When acidic conditions convert deoxyribose to a molecule that binds with diphenylamine to form a
BLUE complex
, Which one of the solutions is the positive control, and which one of the solutions is the negative control
for the Dische reaction? - ✔✔The positive control is the #1 DNA solution and the negative control is
the #4 distilled water.
Do DNA and RNA react alike? Why or why not? - ✔✔No, because DNA lacks an oxygen while RNA
doesn't and this allows it to bind the diphenylamine.
Osmosis - ✔✔The process in which water molecules diffuse across a membrane until
the concentration of water inside and outside the cell is equalized.
Water moves from the area of higher concentration to the area of lower concentration
Moves from lower solute concentration to higher solute concentration
Isotonic solution - ✔✔The same concentration of solute
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