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BIO105 Ch.15 Test Questions & 100% Correct Answers 2024/25 Version

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What is the function of DNA polymerase III? - to add nucleotides to the end of a growing DNA strand What is the function of topoisomerase? - relieving strain in the DNA ahead of the replication fork Which of the following covalently connects segments of DNA? primase DNA polymerase III DNA pol...

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BIO105 Ch.15 Test Questions & 100%
Correct Answers 2024/25 Version
What is the function of DNA polymerase III? - ✔✔to add nucleotides to the end of a growing
DNA strand

What is the function of topoisomerase? - ✔✔relieving strain in the DNA ahead of the replication fork

Which of the following covalently connects segments of DNA?

primase

DNA polymerase III

DNA polymerase I

ligase

helicase - ✔✔ligase

Which of the following help(s) to hold the DNA strands apart while they are being replicated? -
✔✔single-strand DNA binding proteins

You briefly expose bacteria undergoing DNA replication to radioactively labeled nucleotides. When you
centrifuge the DNA isolated from the bacteria, the DNA separates into two classes. One class of labeled
DNA includes very large molecules (thousands or even millions of nucleotides long), and the other
includes short stretches of DNA (several hundred to a few thousand nucleotides in length). These two
classes of DNA probably represent - ✔✔leading strands and Okazaki fragments.

Which of the following synthesizes short segments of RNA? - ✔✔primase

Mendel and Morgan did not know about the structure of DNA; however, which of the following of their
contributions was (were) necessary to Watson and Crick? - ✔✔the particulate nature of the
hereditary material

Put the following steps of DNA replication in chronological order.

, 1. Single-stranded binding proteins attach to DNA strands.

2. Hydrogen bonds between base pairs of antiparallel strands are broken.

3. Primase binds to the site of origin.

4. DNA polymerase binds to the template strand.

5. An RNA primer is created. - ✔✔2, 1, 3, 5, 4

Suppose you are provided with an actively dividing culture of E. coli bacteria to which radioactive
thymine has been added. What would happen if a cell replicates once in the presence of this radioactive
base? - ✔✔DNA in both daughter cells would be radioactive

To repair a thymine dimmer by nucleotide excision repair, in which order do the necessary enzymes act?
- ✔✔endonuclease, DNA polymerase I, DNA ligase

Which of the following statements describes the eukaryotic chromosome?

A) It is composed of DNA alone.

B) The nucleosome is its most basic functional subunit.

C) The number of genes on each chromosome is different in different cell types of an
organism.

D) It consists of a single linear molecule of double-stranded DNA.

E) Active transcription occurs on heterochromatin. - ✔✔D) It consists of a single linear molecule
of double-stranded DNA.

Researchers found E. coli that had mutation rates 100 times higher than normal. Which of the following
is the most likely cause of these results? - ✔✔The proofreading mechanism of DNA polymerase was
not working properly.

What determines the nucleotide sequence of the newly synthesized strand during DNA replication? -
✔✔the nucleotide sequence of the template strand

What is a telomere? - ✔✔the ends of linear chromosomes

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