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ACS Biochemistry Final Exam Study Questions and Answers UPDATED 2024

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  • ACS Biochemistry
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  • ACS Biochemistry

pKa (chapter 3) - measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) - compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) - dipolar ion with both positive and negative groups bu...

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  • August 29, 2024
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  • 2024/2025
  • Exam (elaborations)
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  • ACS Biochemistry
  • ACS Biochemistry
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ACS Biochemistry Final Exam Study
Questions and Answers UPDATED 2024



BESTZONE
ACS BIOCHEM
8/29/24

, ACS Biochemistry Final Exam

pKa (chapter 3) - measure of the tendency of a group to give up a proton (acidity);
tendency decreases tenfold as pKa increases by one unit


thioester bond (1) - compounds with functional group C-S-CoA-C (eg, in acetyl-CoA)


zwitterion (3) - dipolar ion with both positive and negative groups but overall neutral
charge; can act as either an acid or base


Isoelectric point (pI) (chapter 3) - the characteristic pH at which the net electric charge is
zero


SDS - Sodium Dodecyl Sulfate (chap 3) - detergent used to unfold proteins and give
them uniform negative charge


SDS Page (3) - chromatography used to separate proteins based on mass. light
proteins travel fast than heavier ones


PCR - Polymerase chain reaction (3) - copies DNA multiple times to increase sample
size


Isoelectric focusing (chapt 3) - procedure used to determine the isoelectric pt (pI) of a
protein. Protein migrates through gel until pH = pI (net charge = 0)


Two-Dimensional Electrophoresis (3) - combines isoelectric focusing and SDS
electrophoresis; separates proteins by both molecular weight and pI


specific activity (3) - number of enzyme units per mg of total protein (a measure of
enzyme purity)


activity (3) - total units of a certain enzyme in a solution



ACS Biochemistry Final Exam

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