clinical chemistry final exam
Which one of the following is a monosaccharide?
a. lactose
b. fructose
c. sucrose
d. glycogen correct answers Fructose
which of the following are optimal fasting levels for cholesterol based on the NCEP's
adult panel III guidelines?
1. total cholesterol < 250 mg/dL
2. LDL < 100 mg/dL
3. HDL > 60 mg/dL
4. TG < 100 mg/dL
a. 1, 2, 4
b. 2, 3, 4
c. 2, 3
d. 5
e. all are the optimal levels recommended by the NCEP correct answers 2, 3
monosaccharides are connected by ____ in polysaccharides. correct answers
glycosidic bonds
the following laboratory data is received after performing a lipid profile. This data is most
closely associated with which of the following lipoprotein electrophoresis patterns?
standing plasma test = fat layer; clear plasma
total cholesterol = 202 mg/dL
triglycerides = 329 mg/dL
HDL = 36 mg/dL correct answers increased beta and pre-beta lipoprotein with bridging
Which of the following lipoprotein profiles would be indicative of the laboratory
presentation of Tangier disease? correct answers normal VLDL, normal LDL, absent
HDL
A 2 year old girl with hyperlipoproteinemia has the following lipid profile:
cholesterol: 300 mg/dL
LDL : 238 mg/dL
HDL: 35 mg/dL
Triglycerides: 132 mg/dL
standing plasma test: clear
,the above lipoprotein profile and presentation is most consistent with which of the
following lipid pathologies? correct answers hyperbetalipoproteinemia
in addition to hyperglycemia as seen with all types of diabetes (T1DM, T2DM, GDM),
which of the following are other common indicators seen in all types of diabetes
mellitus?
1. glycosuria
2. polyuria
3. hyperinsulinemia
4. ketosis correct answers 1, 2
which of the following metabolic pathways functions to provide glucose for release to
other cells or tissues?
1. glycogenesis
2. glycogenolysis
3. glycolysis
4. gluconeogenesis correct answers 2, 4
a person who has a 2-hour oral glucose tolerance test result of 173 mg/dL would be
classified in which of the following categories? correct answers impaired glucose
tolerance
the following results are from a 21-year-old patient with a back injury who appears
otherwise healthy:
whole blood glucose: 84 mg/dL
serum glucose: 98 mg/dL
CSF glucose: 50 mg/dL
the best interpretation of these results is that: correct answers all values are consistent
with a normal healthy individual
A 30 year old pregnant female has a gestational diabetes mellitus screening test
performed at 26 weeks gestation. Her physician chooses to order a 50g oral glucose
load. Her serum glucose level is 150 mg/dL at 1 hour. What should occur next? correct
answers this is suspicious of diabetes mellitus; an oral glucose tolerance test should be
performed
a 40 year old healthy male named John went to his physician for a well checkup. He is
relatively active (runs 2 miles 4 times per week), eats a balanced diet, has a low stress
job, a normal blood pressure, he does not smoke, and has no history of CHD in his
family. John has no significant medical history and is not on any medications. The
following results were received regarding a risk assessment for CHD.
,total cholesterol: 181.2 mg/dL
HDL: 67.5 mg/dL
triglycerides: 135.3 mol/L
glucose: 70 mg/dL
the calculated LDL in mg/dL is [a] and this is considered [b] for a person with his
number of risk factors. correct answers a. 87 mg/dL
b. normal
this is a 33 year old male investment account executive who says that he feels in
excellent condition. He plays tennis with friends and clients every Sunday and claims to
be victorious most of the time. He is 15-20 pounds over the upper limit of his ideal
weight based on his height, and at times is a chain smoker. His lipid profile results are:
cholesterol: 230 mg/dL
HDL: 25 mg/dL
triglycerides: 150 mg/dL
Apo A1: 0.95 g/L
Apo B: 1.51 g/L
choose all the factors which increase the risk of heart disease: correct answers male,
borderline cholesterol, low HDL, chain smoker
Which of the following enzymes is found bound to HDL in blood plasma and acts to
convert free cholesterol into cholesteryl esters? correct answers Lecithin cholesterol
acyltransferase (LCAT)
you are performing a glycated hemoglobin procedure using ion exchange
chromatography and receive the results below.
A glycated for the standard: 0.480
A total for the standard: 0.575
A glycated for the unknown: 0.420
A total for the unknown: 0.750
C std: 10%
ratio=A glycated/A total
Using the ratio formula above and Beer's law, the calculated glycated hemoglobin of the
unknown sample is: correct answers
All of the following are sample requirements for the determination of total cholesterol
EXCEPT:
serum or plasma (EDTA)
12-16 hours fast
, no illness
no alcohol correct answers 12-16 hour fast ??
following ultracentrifugation pf plasma from a healthy non-diseased person who fasted
16 hours, which of the following fractions will float on top? correct answers LDL
select the order of mobility of lipoproteins after electrophoresis on cellulose acetate or
arose gel at pH 8.6.
1. (-) chylo, pre-B, B, alpha (+)
2. (-) B, pre-B, alpha, Chylo (+)
3. (-) chylo, B, pre-B, alpha (+)
4. (-) alpha, B, pre-B, chylo (+) correct answers 3. (-) chylo, B, pre-B, alpha (+)
Lp(a) is significant when elevated in serum because it: correct answers confers risk for
the development of atherosclerosis
The chemical composition of HDL cholesterol corresponds to: correct answers C
An EDTA blood sample is collected from a nonfasting person for a CBC. Fifteen
minutes after the sample arrives to the laboratory, the physician calls you and
requests a lipid profile including analysis of triglycerides, total cholesterol, HDL, and
LDL be performed on that sample. The physician asks whether or not this can be
done. Your reply would be: correct answers I can perform the total cholesterol and HDL
only
Provided the lipoprotein electrophoresis pattern below from a fasting sample. Which of
the following lipoprotein results would you anticipate? correct answers increased LDL,
decreased VLDL and HDL
Which of the following mechanisms accounts for the elevated plasma level of beta-
lipoproteins seen in hyperbetalipoproteinemia? correct answers ApoB100 receptor
defect
which enzyme deficiency is most commonly associated with hyperchylomicronemia?
correct answers lipase deficiency
which of the following os considered to be a lipid? correct answers cholesterol
John Smithers (21 year old) is in to see his physician for a pre-college physical and
checkup. John has always been extremely healthy. You receive the following lab results
while performing a cholesterol analysis on this patient:
A standard: 0.679
A control: 0.650
A Smithers: 0.729
