BCH210 TESTED QUESTIONS WITH REVISED ANSWERS – UPDATED
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Course
BCH210
Institution
BCH210
BCH210 TESTED QUESTIONS WITH REVISED ANSWERS – UPDATED
cross linker can be used in what techniques - Answer-stabilize X ray structure
limitation of BiOID - Answer-can only proximate, maybe the proteins are just passing by the POI
beads connect to what in BiOid - Answer-biotin released by...
BCH210 TESTED QUESTIONS
WITH REVISED ANSWERS –
UPDATED
cross linker can be used in what techniques - Answer-stabilize X ray structure
limitation of BiOID - Answer-can only proximate, maybe the proteins are just passing by
the POI
beads connect to what in BiOid - Answer-biotin released by ligase
what is PDI - Answer-protein disulfide isomerase
a kind of chaperon, isomerase
structure of DE at pKa, why - Answer-C=O no - charge because protonated
are cofactors proteins - Answer-no
what is heme - Answer-prothetic organic cofactors, bind tightly, shuttle O2
why N S are beta turn AA - Answer-their R group can be modified for PTM
describe zinc finger - Answer-is a motif, zinc is the ligand, bind 2 cys and 2 his, for
stabilizing and DNA recognition
chaperon binds to where and has what property - Answer-hydrophobic, amphipathic
two sites of enzyme - Answer-active-binds substrate
allosteric- binds to other for conformational change
basic and acid are hydrophilic - Answer-si
imidazole is an aromatic ring - Answer-si
mass spectrometry can only be used on where - Answer-known peptides
Dialysis prefer what proteins - Answer-large
affinity chromatography, what is the bond between resin and ligand, ligand and POI -
Answer-covalent noncovalent
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