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BCHM 270 EXAM QUESTIONS WITH CORRECT ANSWERS

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BCHM 270 EXAM QUESTIONS WITH CORRECT ANSWERS A physician would like to evaluate the elevated amounts of a gene being expressed in two different types of tumor cells in order to develop a personalized medicine. Which of the following techniques would be the most appropriate for this purpose? A. W...

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  • August 30, 2024
  • 18
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • bchm 270
  • bchm 270 exam questions with correct answers

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BCHM 270 EXAM QUESTIONS WITH
CORRECT ANSWERS
A physician would like to evaluate the elevated amounts of a gene being expressed in
two different types of tumor cells in order to develop a personalized medicine. Which of
the following techniques would be the most appropriate for this purpose?
A. Western Blot
B. Southern Blot
C. Northern Blot
D. Eastern Blot
E. enzymatic assay - Answer-C

A restriction enzyme can be used for which techniques?
A. Southern blots and cloning.
B. western blots and enzyme assays.
C. Polymerase Chain Reaction and cDNA microarray.
D. Northern blots and SDS-PAGE.
E. ELISA and qPCR. - Answer-A

HindIII is a restriction endonuclease commonly used to cut human DNA into pieces
before inserting it into a plasmid. Which of the following is most likely to be the
recognition sequence for this enzyme?
A. AAGTTC
B. AAGCTT
C. AAGAGA
D. AAGAAG
E. AAGGAA - Answer-B

Protein synthesis:
A. Involves the translation of mRNA into protein.
B. Has the codon CTA as the first amino acid translated.
C. Occurs in the nucleus
D. Does not require ATP and GTP as energy sources.
E. is the transcription of DNA to protein - Answer-A

The best technique to accurately quantify the amount of a specific protein in a sample
is:
A. Northern blot
B. ELISA
C. Southern blot
D. enzyme activity assay
E. PCR - Answer-

,The polymerase chain reaction (PCR):
1.Involves denaturing the target DNA.
2.Involves annealing of two primers to single stranded DNA.
3.Involves chain extension with DNA polymerase.
4.May be used for forensic analysis of DNA samples.

A. 1 and 3 are correct.
B. All are correct.
C. 1, 2, and 3 are correct.
D. 2 and 4 are correct.
E. 4 is correct. - Answer-B

Translation:
1.Describes the synthesis of proteins.
2.Requires amino acid
3.Requires mRNA
4.Requires RNA polymerase

A. If 1, 2, and 3 are correct.
B. If 2 and 4 are correct.
C. If only 4 is correct.
D. If 1 and 3 are correct.
E. If all are correct. - Answer-A.

The mutation most likely to cause damage to protein function in this list is:
A. A mutation in the third position of Thr's ACA codon.
B. The mutation of ATG to AGG in the first codon.
C. A frameshift mutation near the end of the protein sequence.
D. A mutation of CUC to AUC (Leu to Ile) in the middle of the protein. - Answer-B

A molecular biologist has digested DNA with AgeI, which cuts sites 5' A/CCGGT 3' and
the reverse complement sequence 3' TGGCC/A 5'. Which restriction enzyme produces
compatible sticky ends so they can be combined with DNA ligase for cloning?

5' A/CCGGT 3'
3' TGGCC/A 5'

A. SmaI 5' CCC/GGG 3'
B. AvaI 5' C/CCGGG 3'
C. KasI 5' G/GCGCC 3'
D. EaeI 5' T/GGCCA 3' - Answer-

A 39 year old woman is brought to the emergency room complaining of dizziness. She
recalls getting up early that morning to do as much work as possible and had skipped
breakfast. She drank a cup of coffee for lunch and had nothing to eat during the day.
She met with friends at 8pm and had a drink at the bar. She soon became dizzy and

, was transported to the hospital. Following examination, the patient was given orange
juice and immediately felt better. Which one of the following best completes the
sentence? The patient has:

A. presence of an insulinoma.
B. elevated glucagon.
C. blood glucose greater than 70mg/dl.
D. elevated liver glycogen
E. elevated insulin. - Answer-B

Hypoglycemia:
may be a medical emergency because the central nervous system has an absolute
requirement for a continuous supply of glucose.
B. is characterized by symptoms including confusion, aberrant behavior, or coma.
C. is resolved within minutes following administration of intravenous glucose.
D. occurs when the blood glucose levels are equal to or less than 40mg/dl.
E. All of the above - Answer-E

Insulin is a peptide hormone:
A. that increases glucose uptake and the synthesis of glycogen, protein, and fat.
B. that is composed of a single protein chain.
C. that is synthesized by the alpha-cells of the islets of Langerhans of the pancreas.
D. whose biosynthesis involves one step resulting in the active hormone being
produced. - Answer-A

Insulin resistance:
A. Is described as a decreased ability of liver, adipose tissue and muscle to respond to
normal blood insulin levels.
B. Always leads to the development of type 2 diabetes.
C. Leads to type 2 diabetes in over 96% of patients presenting with insulin resistance.
D. Is characterized by uncontrolled production of glycogen. - Answer-A

Relative or absolute lack of insulin in humans would result in which of the following
reactions in the liver?
A. Decreased action of hormone-sensitive lipase.
B. Decreased gluconeogenesis from lactate.
C. Increased glycogen synthesis.
D. Increased formation of 3-hydroxybutyrate.
E. Decreased glycogenolysis. - Answer-D

Type 1 diabetes:
A. develops during childhood or puberty.
B. results from b-cell destruction which eliminates insulin production from the pancreas.
C. always requires insulin as a treatment.
results
D. in hyperglycemia and ketoacidosis.

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