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BCHM 270 MIDTERM TEST QUESTIONS WITH CORRECT ANSWERS

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BCHM 270 MIDTERM TEST QUESTIONS WITH CORRECT ANSWERS Intramolecular disulfide bond - Answer-2 C (1 C can form disulfide bonds with other C groups) Phosphorylated - Answer-S, T, Y Alpha helix breakers - Answer-P Bulky clusters: Y, W, L, V, F Charged clusters: K, R, H, D, E Ionizable si...

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  • August 30, 2024
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BCHM 270 MIDTERM TEST QUESTIONS
WITH CORRECT ANSWERS
Intramolecular disulfide bond - Answer-2 C
(1 C can form disulfide bonds with other C groups)

Phosphorylated - Answer-S, T, Y

Alpha helix breakers - Answer-P
Bulky clusters: Y, W, L, V, F
Charged clusters: K, R, H, D, E

Ionizable side chains - Answer-R, K, Y, C, H, E, D

Scurvy - Answer-Caused by impaired hydroxylation of lysine and proline causing
unstable triple helices due to vitamin C deficiency.

Prion disease - Answer-Proteins are refolded from α-helix into β-sheet form, producing
long insoluble fibrils and a loss of function.

Alzheimer's disease - Answer-Abnormal cleavage of the hydrophobic section of a
protein, which causes aggregation into long, insoluble neurotoxic fibrils.

Ostegenesis imperfecta - Answer-Mutation of Gly-X-Y repeating sequences into bulky
amino acids, preventing assembly of helices into a mature collagen fiber (brittle bone
disease).

Respiratory acidosis - Answer-Caused by a sharp increase of carbon dioxide in the
blood.

Sickle cell anemia - Answer-The aggregation of a mutated hemoglobin protein caused
by the substitution of glutamic acid to valine.

Ehlers-Danlos syndrome - Answer-Proteins are refolded from α-helix into β-sheet form,
producing long insoluble fibrils and a loss of function.

Oxidoreductase - Answer-Transfer electrons between molecules; catalyze oxidation-
reduction reactions.

Transferase - Answer-Transfer functional groups between molecules.

Hydrolyase - Answer-Cleaves molecules by the addition of water.

, Lyase - Answer-Adds atoms or functional groups to a double bond or removes them to
form double bonds.

Isomerases - Answer-Move functional groups within a molecule.

Ligases - Answer-Join two molecules in a reaction powered by ATP hydrolysis.

Spontaneous - Answer-Negative ΔG

Requires additional energy - Answer-Positive ΔG

Equilibrium - Answer-ΔG = 0

Exergonic reaction - Answer-Releases energy

Endergonic reaction - Answer-Absorbs energy

Competitive inhibitor - Answer-Binds to substrate-free active site
Vmax doesn't change
Km increases

Uncompetitive inhibitor - Answer-Binds to substrate bound enzyme
Vmax decreases
Km decreases

Noncompetitive inhibitor - Answer-Equal affinity to bind to both substrate bound and free
enzyme
Vmax decreases
Km doesn't change

Mixed inhibitor - Answer-Binds to both substrate bound and free enzyme, but favours
one over other
Vmax decreases
Km increases or decreases

Citrate synthase - Answer-Activated by: N/A
Inhibited by: citrate

Isocitrate dehydrogenase - Answer-Activated by: ADP, Ca2+
Inhibited by: ATP, NADH

α-ketoglutarate dehydrogenase complex - Answer-Activated by: Ca2+
Inhibited by: NADH, succinyl CoA

Protein synthesis:
a) Is the transcription of DNA to protein

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