MICROB 202 FINAL EXAM QUESTIONS AND ANSWERS (100% PASS)
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Course
MICROB 201/ 202
Institution
MICROB 201/ 202
MICROB 202 FINAL EXAM QUESTIONS AND
ANSWERS (100% PASS)
common traits of Streptococcus and Enterococcus species - Answer️️ -- Gram-
positive cocci
- Strepto (chain) arrangement
- do not produce endospores
- aerotolerant
- negative result for catalase assay
catalase assay - Answer️️ ...
MICROB 202 FINAL EXAM QUESTIONS AND
ANSWERS (100% PASS)
common traits of Streptococcus and Enterococcus species - Answer✔️✔️-- Gram-
positive cocci
- Strepto (chain) arrangement
- do not produce endospores
- aerotolerant
- negative result for catalase assay
catalase assay - Answer✔️✔️-tests for the production of catalase to differentiate
between Staph/Micro cells and Strep/Entero cells
catalase - Answer✔️✔️-oxygen-detoxifying enzyme that breaks down hydrogen
peroxide to water and oxygen gas
positive reaction for catalase assay - Answer✔️✔️-bubbles forming in peroxide
solution due to oxygen gas being produced, indicative that species is catalase-
positive
- must be Staphylococcus or Micrococcus
negative reaction for catalase assay - Answer✔️✔️-no bubbles forming in peroxide
solution, indicative that species is catalase-negative
hemolysis on blood agar - Answer✔️✔️-pattern of breaking red blood cells can be
used to differentiate between Streptococcus and Enterococcus species
- can be alpha, beta, or gamma
alpha hemolysis - Answer✔️✔️-partial breakdown of red blood cells; medium will
remain opaque but changes to a greenish-brown color (due to biliverdin pigment);
also called 'green hemolysis'
- viridans streps (Strep species found in oral cavity)
- S. mutans (tooth decay)
- S. pneumonia
beta hemolysis - Answer✔️✔️-species that carry out secrete toxic polypeptides
(streptolysins) that lyse red blood cells; produces slightly yellow-tinted transparent
halo around colonies
- S. pyogenes (strep throat, scarlet fever)
gamma hemolysis - Answer✔️✔️-species does not lyse red blood cells, no
hemolysis has occurred; medium remains red in color
- E. faecalis (UTIs)
Lancefield groups - Answer✔️✔️-identification system of Streptococcus and
Enterococcus species by carbohydrates on the cell's surface; carbs act as antigens
that are detected with antibodies
common traits of Staphylococcus and Micrococcus species - Answer✔️✔️-- Gram-
positive cocci
- do not produce endoscopes
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