Why is it important as a beginning microbiologist to appreciate the ubiquity of microorganisms when
working in a microbiology lab and trying to maintain aseptic technique? - .To be aware that
microorganisms are everywhere and can very easily contaminate your cultures
Identify where you would pla...
BIOL 2420 Lab Practical 1
Why is it important as a beginning microbiologist to appreciate the ubiquity of microorganisms when
working in a microbiology lab and trying to maintain aseptic technique? - .To be aware that
microorganisms are everywhere and can very easily contaminate your cultures
Identify where you would place the culture in the following scenario:
You inoculated a broth with E. coli in lab and want to grow the culture for 48 hours and will check on the
culture during the next lab period. - 37 degrees C
Where and how should used media (culture tubes and plates) be disposed of? - directly into bio
hazard bin
Where and how should a slide prepared by you (ex: Gram stain) be disposed of? - Wrapped in a
paper towel and disposed into the biohazard bin
Where should gloves worn by you (after lab, not cleaning up a spill) be disposed of? - in the
regular trash can
Where should a broken stock slide (ex: 3 forms slide that was observed during lab 3) be disposed of? -
in the broken glass container
What are the last two things that you always do before leaving the microbiology lab that will ensure that
you do not spread organisms used in the lab out of the lab and will also ensure that the next class
coming into the lab will not be contaminated? - 1. disinfect lab bench
2. wash hands
Select all required information needed to properly label your cultures when labeling culture media. -
1. name of medium (TSA, TSB)
2. date of inoculation
3. the inoculum (microbes name)
4. your initials (F, M, L)
,liquid media - media that does not contain agar
solid media - media that contains the solidifying agent, agar, in it
incinerator - Equipment used to sterilize the inoculating loops via incineration
inoculate - Introduce cells or organisms into a culture medium
inoculum - Organism or material used for inoculation
incubator - Equipment used to grow microorganisms in a controlled environment
What is the single most important way to reduce the spread of unwanted microbes? -
handwashing
Why is it incorrect to label a culture tube cap or the lid of the petri dish? - 1. Lids can easily fall off
or be switched.
2. Plate media is incubated upside down
Identify where you would place the culture in the following scenario:
You inoculated a broth with E. coli in lab but did so at the end of the week. You won't be back in lab until
after the weekend. Therefore, the growth needs to be slowed down as the incubation time will be longer
than 72 hours. - 25 degrees C
Identify where you would place the culture in the following scenario:
You have observed your results but want to store your culture for future observations. - 4 degrees
C refrigeration
Observe Figure 2-1. Microorganisms are everywhere in the environment. Select two beneficial roles that
microorganisms play in the environment. - 1. decomposers
, 2. producers
Microorganisms are found everywhere on body surfaces and lining lumens. What four beneficial roles do
microorganisms play as microbiota? - 1. exclude pathogens from colonizing
2. aid in digestion
3. Synthesize Vitamins K & B12
4. Stimulate immune response
Select the two steps you need to follow to properly handle the microscope power cord when you are
finished. - 1. Wrap the cord around the microscope base
2.Hold the plug and remove it from the outlet
Select the two steps you need to follow to properly put away prepared stock slides when you are
finished. - 1. Wipe fingerprints and oil off with a paper towel
2. Return slide to designated tray
Select the two steps you need to follow to properly put away student stained slides when you are
finished. - 1. Wrap slide in a paper towel
2. Throw slide away in biohazard container
Select the two steps you need to follow to properly put the microscope away after using oil. - 1.
Dab the end of the lens with lens paper
2. Make sure that oil is not on any of the lenses.
When you are done with the microscope, rotate the revolving nosepiece so that _______ is over the
stage - The scanning objective (4x)
When you are done with the microscope, what position should the stage be in? - as low as it can
go
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