Class notes
Class Notes: Lecture 2 of M118L Course at UCI
- Institution
- University Of California - Irvine
Notes cover lecture 2A and 2B videos of M118L course at UCI. Includes lecture 2's practice problems.
[Show more]
Seller
Follow
Content preview
Lecture 2 (cover videos A & B)Abbreviations:
Tuesday, January 12, 2021 10:21 PM
Ex. = for example,
b/w = between
b/c = because
w/ = with
Bacterial Identification (week 1 notes continued)
LECTURE PRACTICE PROBLEMS
• (After we have isolated bacteria from week 1) Are our single colonies a pure culture?
Catalase/Oxidase tests lets you determine aerobe versus anaerobe. Y or N? No! Whether a
No, bacteria just have enough space on media to grow independently.
bacterium is an anaerobe or aerobe is NOT dependent on these tests! That is determined
• Pure cultures can be identified when all single colonies look similar.
by whether the bacterium can grow without OR with oxygen. -> You cannot assume
• Isolation -> confirm pure culture -> complete Gram stain of the unknowns -> ABA DABA
bacteria is an anaerobe/aerobe from these tests. The catalase/oxidase tests do not tell us if the
tests
organism utilizes O2 in its metabolic pathway.
• ABA DBA test: aides in identifying the family/genus of unknown bacteria. Includes:
○ Catalase test, oxidase test, phenol red test, minimal media test, fluorescent
What is the ABA DABA designation?
pigment test, motility test.
1. Isolate A - Gram positive, rod shaped, oxidase negative, catalase positive, pigment
• How to read any scientific paper:
negative, grows on minimal media, phenol red negative, non-spore forming, motile ->
○ What is the main question the authors are asking (purpose of the paper)?
+AAB. Assumed it was an obligate aerobe b/c it possesses catalase (breaks down O2
○ What is the answer to that question?
radicals by-producted from the ETC) and it does not undergo fermentation (cannot
○ For each figure –What is the purpose of the figure?–What do the authors learn and
ferment glucose).
how does that tie into the main question?
2. Isolate B - Gram negative, cocci, oxidase positive, catalase positive, pigment negative,
does not grow on minimal media, phenol red positive, non-motile -> -ACB. Can ferment
• Scientific Paper 1:
glucose, BUT also possesses cytochrome c oxidase and expresses catalase-- most
○ Spectrophotometer: measures growth of a bacteria. Gives an optical density-- how
likely grows w/ O2.
many cells are detected when light passes thru a sample. Ex. The higher the
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller PrinceAlixD. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $2.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
81531 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now