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Class Notes: Lecture 4 of M118L Course at UCI $2.99   Add to cart
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  3. University Of California - Irvine
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  5. Experimental Microbiology Lab (M118L)

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Class Notes: Lecture 4 of M118L Course at UCI
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Notes cover lecture 4A and 4B videos of M118L course at University of California, Irvine. Includes review of general writing structure of lab reports.

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  • September 1, 2024
  • 3
  • 2020/2021
  • Class notes
  • Brian sato
  • All classes

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Lecture 4 (covers videos A & B) Abbreviations:
Monday, January 25, 2021 11:10 AM Ex. = for example,
b/w = between
b/c = because
w/ = with
v. = versus
INC = increase
A. Identification


• Motility test -> use a wet mount procedure!


○ Live organisms are placed on coverslip, covered in water, and observed.


○ VERY different from Gram stain procedure in that organisms observed in a


Gram stain are dead and fixed.


○ Group movement is force of coverslip pressure, NOT indicative of motility!


○ -> Individual movement is key!


○ Since there is no stain, the slide itself will be marked. Can focus cells and


water in that area.


• Endospore test


○ Green stain is used to identify spores. Safranin-O is used as counterstain. -


> Green spores in pink cells!


○ While performing the spore stain you forget to heat the first step. What do


you expect to see? The spore w/o color and the rest of the cell pink.


Heating is used to soften the spore's protein coat and allow the malachite


green to enter the spore; w/o heating, the dye cannot penetrate the spore in


the first place.



B. Lab Report 1 - Antibiotic Resistance


LAB OBJECTIVE

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