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Class Notes: Lecture 7 of M118L Course at UCI $2.99   Add to cart
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  3. University Of California - Irvine
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  5. Experimental Microbiology Lab (M118L)

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Class Notes: Lecture 7 of M118L Course at UCI
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Notes cover lecture 7A video of M118L course at University of California, Irvine. Covers concepts like comparing aged to un-aged cells in microbiology lab experiments.

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  • September 1, 2024
  • 3
  • 2020/2021
  • Class notes
  • Brian sato
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Lecture 7 (covers video A)
Tuesday, February 16, 2021 11:03 PM




• Aged cells are compared to unaged--same strain, only grown for 1-2d, fresh and metabolically-active-- cells.


Once aged cells show growth advantage, they exhibit the GASP phenotype.


• Hypothesis: is the GASP phenotype present? Does it differ in different media?


• Pipetting expt: determining how many colony-forming units (CFUs) will come up if a mL of culture is diluted from


0,3,6,9 days? CFU is a measurement of concentration.


○ Each spot represents a 10-fold dilution.


○ In earlier spots, there's so much growth cells are merging onto one another-- must look at spot 5+.


○ Aged cells are grown in their LTSP-- 90% of the cells in the growth culture are dead. Shining light onto a


culture full of dead cells and measuring the OD will not determine how many aged cells are still alive/can


grow.


○ Spot 1 is 10ul of the diluted culture (10ul culture + 90ul LB). This means it contains 1ul of the original


culture. ->


○ Spot 1: CFU/10^-3ml = CFU * 10^3/ml

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