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Determine how rxn rate (velocity) varies w/ substrate concentration. Rate increase, Rate
decreases, Rate is unchanged
A. additional substrate is added when substrate concentration is low
B. substrate is added when enzymes is saturated w/ substrate
C. additional substrate is added when substrate concentration is very high - Answer Rate
increases: A
Rate decreases: none
Rate is unchanged: B, C
Match the condition w/ the statement that describe it.
Less than 10% of the active sites are occupied by substrate
A. [S]= 10Km
B. [S]= Km
C. [S]= 0.1Km - Answer C
Velocity or rate of rxn is defined as - Answer rate of decrease of the reactant or increase
of product with time
first order rxn - Answer when the velocity of a rxn (units M/s) is linearly proportional to
the reactant concentration (unit M or mole/L)
First order rxn formula - Answer V= k[A]
k value of first/second order rxns - Answer rate constant
, __________ order rxns can appear to be first order rxn if concentration of one reactant
is much higher than the other - Answer Second
Pseudo-first order - Answer second order rxns can appear to be first order rxn if
concentration of one reactant is much higher than the other
Second order rxn formula - Answer V= k[A]^2 or V= k[A][B]
zero order rxn - Answer rxn rate is independent of reactant concentration under some
specific conditions
Investigating enzyme kinetics is to measure... - Answer velocity of rxn as a function of
substrate concentration [S] w/ a fixed amount of enzyme [E]t
In a catalyzed rxn,_____ increases at low [S] then levels off - Answer [V]o
Leonor Michaelis and Maud Menten derived an equation to describe ... - Answer the
initial rxn velocity, Vo, as a function of substrate concentration [S} for enzymatic
catalyzed rxn, called Michaelis-Menten equation
Michaelis-Menten equation - Answer Vo= Vmax * [S]/ [S]+Km
Vmax= - Answer k2[E]t
Km= - Answer (k-1 + k2) / k2