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Biochemistry ACS Exam B Questions and Answers Fully Solved
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Biochemistry ACS Exam B
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Biochemistry ACS Exam BGlycolysis - answer Near-universal pathway in which glucose molecule is oxidized to
two molecules of pyruvate, with energy conserved as ATP and NADH
Glycolytic breakdown - answer Glucose is the sole source of metabolic energy in some
mammalian tissues and cell types
Fermentation - answer general form for the anaerobic degradation of glucose or other
organic nutrients to obtain energy, conserved as ATP
Preparatory phase - answerATP is invested to convert glucose to fructose 1,6-
bisphosphate.
C3 & C4 broken to yield two molecules of triose phosphate.
Glycolysis: Preparatory phase - answer1. Glucose phosphorylated at OH on C-6, ATP
used
*hexokinase*
2. glucose 6-phosphate thus formed is converted to fructose 6-phosphate.
*phosphohexose isomerase*
3. Fructose 6-phosphate is phorylated at C-1 to yield Fructose
1,6-bisphosphate, ATP used
*PFK-1*
4. Fructose 6-phosphate splits to 2 three carbon molecules, DHAP and GAP
*aldolase*
5. DHAP isomerize to GAP
*triose phosphate isomerase*
(2 GAP molecules)
Glycolysis: Payoff phase-basic def. - answerThe energy of ATP is invested, raising the
free-energy content of the intermediates, and the carbon chains of all the metabolized
hexoses are converted to a common GAP (glyceraldehyde 3-phosphate)
Yields 2 ATP and NADH per triose phosphate oxidized.
Glycolysis: Payoff phase - answer6. GAP is oxidized and phosphorylated by inorganic
phosphate (not by ATP) to form 1,3-bisphosphoglycerate
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